Aims

Pseudomonas koreensis, a subgroup of the P. fluorescens complex, is a potential plant growth-promoting rhizobacterium. This study explored the mechanisms of plant growth promotion by P. koreensis and its potential to induce stress tolerance in tomato.

Methods

Tomato plants in pots were inoculated with P. koreensis GS and cultured for 60 days. RNA sequencing and gas chromatography-mass spectrometry were used to detect global transcriptomic and metabolomic changes in tomato leaves. Liquid chromatography-mass spectrometry was used for quantification of plant hormones.

Results

The inoculated plants showed more vigorous growth, with higher leaf chlorophyll content and shoot biomass compared with uninoculated controls. The activities of several defense enzymes (e.g., phenylalanine ammonia-lyase) were enhanced in the leaves of inoculated plants. A total of 737 differentially expressed genes were identified, which were related to plant hormone biosynthesis, MAPK signaling transduction, and polyphenol biosynthesis. The contents of specific metabolites related to plant growth and stress tolerance, including polyphenols (phenylpropanoids) and amino acids (tryptophanand and proline), were increased after inoculation. Plant hormones such as indole-3-acetic acid participated in plant growth promotion by P. koreensis.

Conclusions

P. koreensis promoted plant growth and showed potential to induce stress tolerance in tomato by enhancing auxin and polyphenol-related pathways.

中文翻译：

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韩国假单胞菌可促进番茄生长并显示出通过生长素和多酚相关途径诱导胁迫耐受性的潜力

目的

假单胞菌（Pseudomonas koreensis）是荧光假单胞菌（P.fluorescens）复合体的一个亚组，是一种潜在的促进植物生长的根瘤菌。本研究探讨了朝鲜红杆菌促进植物生长的机制及其诱导番茄耐逆性的潜力。

方法

在盆中的番茄植株上接种韩国假单胞菌GS，并培养60天。RNA测序和气相色谱-质谱法用于检测番茄叶片的总体转录组和代谢组学变化。液相色谱-质谱法用于定量植物激素。

结果

与未接种的对照相比，接种后的植物生长更旺盛，叶绿素含量和茎生物量更高。接种植物叶片中几种防御酶（例如苯丙氨酸氨解酶）的活性增强。共鉴定出737个差异表达基因，这些基因与植物激素生物合成，MAPK信号转导和多酚生物合成有关。接种后，与植物生长和胁迫耐受性相关的特定代谢物的含量增加，其中包括多酚（苯丙烷）和氨基酸（色氨酸和脯氨酸）。吲哚-3-乙酸等植物激素参与了红假单胞菌对植物生长的促进作用。

结论

P. koreensis促进植物生长，并显示出通过增强生长素和多酚相关途径来诱导番茄抗逆性的潜力。