Acinetobacter baumannii, as a nosocomial pathogen has become a worldwide concern in recent years. In the current study, the resistance to tetracyclines and colistin were assessed in the isolates from different provinces of Iran.During the timeline of this study, a number of 270 isolates of A. baumannii were collected from tracheal aspirates, wounds, urine and blood cultures. The minimum inhibitory concentration (MIC) for tetracycline, doxycycline, minocycline, tigecycline and colistin were evaluated. Tetracycline resistance genes were assessed by PCR. The mean expression level of adeB, adeJ and adeG were assessed using semi quantitative Real-Time PCR. The clonal relationship of the isolates was evaluated by the repetitive extragenic palindromic PCR (REP-PCR), International Clonal (IC) Lineage Multiplex PCR and multilocus sequence typing (MLST) (Pasteur scheme) methods.The MIC by microdilution method showed that 87.5, 51.4, 28, 0.74 and 0% of the isolates were resistant to tetracycline, doxycycline, minocycline, tigecycline and colistin respectively. The prevalence of tetracycline resistance genes was 99.2, 99.2, 98, 86.7, 10, 3.33, 0.37, 0% for adeB, adeJ, adeG, tetB, tetA(39), tetA, tetM and tetH in tetracycline-resistant isolates. Moreover, the expression level of adeB, adeJ, adeG genes in tigecycline-nonsusceptible A. baumannii (TNAB) strain was higher compared to the tigecycline-susceptible A. baumannii (TSAB). A broad genomic diversity was revealed, but ST2 was the most prevalent ST. Our results indicated that tetracycline resistance in Iran is mediated by resistance-nodulation-cell division (RND) and tetB efflux pumps.

中文翻译：

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伊朗多重耐药鲍曼不动杆菌 ST2 外排泵基因的检测。

鲍曼不动杆菌作为一种院内病原菌，近年来已成为全球关注的焦点。在目前的研究中，对来自伊朗不同省份的分离株对四环素和粘菌素的耐药性进行了评估。在本研究的时间线中，从气管抽吸物、伤口、尿液和血液培养物中收集了 270 份鲍曼不动杆菌分离株. 评估了四环素、强力霉素、米诺环素、替加环素和粘菌素的最低抑菌浓度（MIC）。通过PCR评估四环素抗性基因。使用半定量实时 PCR 评估 adeB、adeJ 和 adeG 的平均表达水平。通过重复基因外回文PCR（REP-PCR）评估分离物的克隆关系，国际克隆 (IC) 谱系多重 PCR 和多位点序列分型 (MLST)（巴斯德方案）方法。微量稀释法的 MIC 表明，87.5、51.4、28、0.74 和 0% 的分离株对四环素、强力霉素、米诺环素、分别为替加环素和多粘菌素。adeB、adeJ、adeG、tetB、tetA(39)、tetA、tetM和tetH在四环素耐药株中四环素耐药基因的流行率为99.2、99.2、98、86.7、10、3.33、0.37、0%。此外，与替加环素敏感的鲍曼不动杆菌（TSAB）相比，替加环素不敏感的鲍曼不动杆菌（TNAB）菌株中adeB、adeJ、adeG基因的表达水平更高。揭示了广泛的基因组多样性，但 ST2 是最普遍的 ST。