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Capture and detection of urine bacteria using a microchannel silicon nanowire microfluidic chip coupled with MALDI-TOF MS
Analyst ( IF 4.2 ) Pub Date : 2021-1-18 , DOI: 10.1039/d0an02222e
Yuexin Li 1, 2, 3, 4 , Tao Wang 1, 2, 3, 4 , Jianmin Wu 1, 2, 3, 4
Affiliation  

Fast bacterial identification in urine samples was achieved by capturing bacteria on a microchannel silicon nanowire microfluidic chip, followed by MALDI-TOF MS detection. Under the optimized conditions, bacteria with a concentration of 106 CFU mL−1 in urine samples could be identified without culture. If cultured for 4 hours, bacteria with a concentration as low as 103 CFU mL−1 were identified.

中文翻译:

使用微通道硅纳米线微流控芯片结合MALDI-TOF MS捕获和检测尿液细菌

通过在微通道硅纳米线微流控芯片上捕获细菌,然后进行MALDI-TOF MS检测,可以快速识别尿液样品中的细菌。在最佳条件下,无需培养即可鉴定尿液样品中浓度为10 6 CFU mL -1的细菌。如果培养4小时,则鉴定出浓度低至10 3 CFU mL -1的细菌。
更新日期:2021-02-03
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