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Two-dimensional (2D) dynamic vibration optical coherence elastography (DV-OCE) for evaluating mechanical properties: a potential application in tissue engineering
Biomedical Optics Express ( IF 3.4 ) Pub Date : 2021-02-03 , DOI: 10.1364/boe.416661 Hsiao-Chuan Liu 1 , Piotr Kijanka 2 , Matthew W Urban 1, 3
Biomedical Optics Express ( IF 3.4 ) Pub Date : 2021-02-03 , DOI: 10.1364/boe.416661 Hsiao-Chuan Liu 1 , Piotr Kijanka 2 , Matthew W Urban 1, 3
Affiliation
Mechanical properties in tissues are an important indicator because they are associated with disease states. One of the well-known excitation sources in optical coherence elastography (OCE) to determine mechanical properties is acoustic radiation force (ARF); however, a complicated focusing alignment cannot be avoided. Another excitation source is a piezoelectric (PZT) stack to obtain strain images via compression, which can affect the intrinsic mechanical properties of tissues in tissue engineering. In this study, we report a new technique called two-dimensional (2D) dynamic vibration OCE (DV-OCE) to evaluate 2D wave velocities without tedious focusing alignment procedures and is a non-contact method with respect to the samples. The three-dimensional (3D) Fourier transform was utilized to transfer the traveling waves (x, y, t) into 3D k-space (kx, ky, f). A spatial 2D wavenumber filter and multi-angle directional filter were employed to decompose the waves with omni-directional components into four individual traveling directions. The 2D local wave velocity algorithm was used to calculate a 2D wave velocity map. Six materials, two homogeneous phantoms with 10 mm thickness, two homogeneous phantoms with 2 mm thickness, one heterogeneous phantom with 2 mm diameter inclusion and an ex vivo porcine kidney, were examined in this study. In addition, the ARF-OCE was used to evaluate wave velocities for comparison. Numerical simulations were performed to validate the proposed 2D dynamic vibration OCE technique. We demonstrate that the experimental results were in a good agreement with the results from ARF-OCE (transient OCE) and numerical simulations. Our proposed 2D dynamic vibration OCE could potentially pave the way for mechanical evaluation in tissue engineering and for laboratory translation with easy-to-setup and contactless advantages.
中文翻译:
用于评估机械性能的二维 (2D) 动态振动光学相干弹性成像 (DV-OCE):在组织工程中的潜在应用
组织中的机械特性是一个重要指标,因为它们与疾病状态有关。光学相干弹性成像 (OCE) 中用于确定机械性能的众所周知的激发源之一是声辐射力 (ARF);然而,无法避免复杂的对焦对准。另一个激发源是压电 (PZT) 堆栈,通过压缩获得应变图像,这会影响组织工程中组织的内在机械性能。在这项研究中,我们报告了一种称为二维 (2D) 动态振动 OCE (DV-OCE) 的新技术,无需繁琐的聚焦对准程序即可评估 2D 波速度,并且是一种非接触式样品方法。三维 (3D) 傅立叶变换用于传输行波 (X,Y,T)转换成3D ķ k-空间(ķ X,K ÿ,F)。采用空间二维波数滤波器和多角度定向滤波器将具有全向分量的波分解为四个单独的行进方向。二维局部波速算法用于计算二维波速图。六种材料,两个 10 毫米厚的同质体模,两个 2 毫米厚的同质体模,一个直径为 2 毫米的异质体模和一个离体本研究对猪肾进行了检查。此外,ARF-OCE 用于评估波速以进行比较。进行了数值模拟以验证所提出的二维动态振动 OCE 技术。我们证明实验结果与 ARF-OCE(瞬态 OCE)和数值模拟的结果非常一致。我们提出的二维动态振动 OCE 可能为组织工程中的机械评估和实验室翻译铺平道路,具有易于设置和非接触的优势。
更新日期:2021-03-01
中文翻译:
用于评估机械性能的二维 (2D) 动态振动光学相干弹性成像 (DV-OCE):在组织工程中的潜在应用
组织中的机械特性是一个重要指标,因为它们与疾病状态有关。光学相干弹性成像 (OCE) 中用于确定机械性能的众所周知的激发源之一是声辐射力 (ARF);然而,无法避免复杂的对焦对准。另一个激发源是压电 (PZT) 堆栈,通过压缩获得应变图像,这会影响组织工程中组织的内在机械性能。在这项研究中,我们报告了一种称为二维 (2D) 动态振动 OCE (DV-OCE) 的新技术,无需繁琐的聚焦对准程序即可评估 2D 波速度,并且是一种非接触式样品方法。三维 (3D) 傅立叶变换用于传输行波 (X,Y,T)转换成3D ķ k-空间(ķ X,K ÿ,F)。采用空间二维波数滤波器和多角度定向滤波器将具有全向分量的波分解为四个单独的行进方向。二维局部波速算法用于计算二维波速图。六种材料,两个 10 毫米厚的同质体模,两个 2 毫米厚的同质体模,一个直径为 2 毫米的异质体模和一个离体本研究对猪肾进行了检查。此外,ARF-OCE 用于评估波速以进行比较。进行了数值模拟以验证所提出的二维动态振动 OCE 技术。我们证明实验结果与 ARF-OCE(瞬态 OCE)和数值模拟的结果非常一致。我们提出的二维动态振动 OCE 可能为组织工程中的机械评估和实验室翻译铺平道路,具有易于设置和非接触的优势。