In the present study, a precharacterized oleaginous microalgal species (Chlorella vulgaris) was exploited for its biomass productivity, lipid yield, and fatty acid analysis under mixotrophic and photoautotrophic conditions. For the purpose, the microalgal biomass was raised mixotrophically as well photoautotrophically. For mixotrophic cultivation, molasses was used as additional source of carbon and energy, while for photoautotrophic cultivation, atmospheric CO₂ was the sole source of carbon and energy. The microalgal biomass raised thus was then harvested following 5 days of nitrogen starvation. Mixotrophic cultivation depicted remarkably higher biomass productivity (137.43 ± 13.3 mg L⁻¹ day⁻¹) than that of photoautotrophic cultivation (91.57 ± 7.9 mg L⁻¹ day⁻¹). About 39% (w/w) of total lipid content was obtained from the dried biomass raised mixotrophically. Lipidomic analysis of the extracted oil depicted higher concentrations of polyunsaturated fatty acids (palmitic and oleic acids). The significant availability of polyunsaturated fatty acids (60 mg g⁻¹ of dried algal biomass) ensured the production of high quality biofuel.

中文翻译：

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氮饥饿前甘蔗糖蜜中小球藻的混合营养培养：生物质生产力、脂质含量和脂肪酸分析

在本研究中，在混合营养和光合自养条件下，利用一种预先表征的产油微藻（小球藻）的生物量生产力、脂质产量和脂肪酸分析。为此，微藻生物质以混养方式以及光自养方式进行培养。对于混合营养培养，糖蜜被用作额外的碳和能量来源，而对于光合营养培养，大气 CO ₂是碳和能量的唯一来源。然后在氮饥饿5天后收获由此产生的微藻生物质。混合营养培养显示出显着更高的生物质生产力（137.43 ± 13.3 mg L ^-1 天^-1) 比光合自养培养 (91.57 ± 7.9 mg L ^-1 天^-1 )。大约 39% (w/w) 的总脂质含量是从混合营养培养的干燥生物质中获得的。提取油的脂质组学分析表明多不饱和脂肪酸（棕榈酸和油酸）浓度更高。多不饱和脂肪酸的显着可用性（60 mg g ^-1干藻类生物质）确保了高质量生物燃料的生产。