Codium fragile is a green alga belonging to Codiales family. The sulfated polysaccharides of this alga have anti‐coagulation, antiviral, anti‐angiogenesis, antioxidant, and immunoregulatory properties. In this study, we developed a reliable and rapid method for the analysis of 10 monosaccharides using ultra‐performance liquid chromatography–tandem mass spectrometry in the negative electrospray ionization and multiple reaction monitoring mode. Monosaccharides, including two pentoses (xylose, arabinose); two deoxyhexoses (rhamnose, fucose); three hexoses (mannose, glucose, galactose); two hexuronic acids (glucuronic acid, galacturonic acid), and an N‐acetyl‐hexosamine (glucosamine), were derivatized using 1‐phenyl‐3‐methyl‐5‐pyrazolone and simultaneously analyzed within 9 min. Optimization of the derivatization process, especially by using various 1‐phenyl‐3‐methyl‐5‐pyrazolone concentrations, was studied. The calibration curves showed good linearity with a squared correlation coefficient > 0.995. The spiked recovery was determined to be 91.1–105.7% with the relative intra‐day and inter‐day standard deviations ranging from 2.58–6.71% and 3.15–7.67%, respectively. The limit of detection and limit of quantification for all 10 monosaccharides ranged from 0.02 to 0.10 μg/mL and 0.05 to 0.25 μg/mL, respectively. Using this method, the monosaccharides comprising the polysaccharides of Codium fragile were determined to be arabinose, galactose, and glucose.

中文翻译：

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超高效液相色谱-串联质谱法分析脆鳕水溶性多糖的单糖组成

易碎的鳕鱼是属于科迪亚莱斯科的绿藻。这种藻类的硫酸化多糖具有抗凝血、抗病毒、抗血管生成、抗氧化和免疫调节特性。在本研究中，我们开发了一种使用超高效液相色谱-串联质谱在负电喷雾电离和多反应监测模式下分析 10 种单糖的可靠且快速的方法。单糖，包括两种戊糖（木糖、阿拉伯糖）；两种脱氧己糖（鼠李糖，岩藻糖）；三种己糖（甘露糖、葡萄糖、半乳糖）；使用 1-苯基-3-甲基-5-吡唑啉酮衍生两种己糖醛酸（葡萄糖醛酸、半乳糖醛酸）和一种 N-乙酰己糖胺（葡萄糖胺），并在 9 分钟内同时进行分析。衍生化过程的优化，特别是通过使用不同浓度的 1-苯基-3-甲基-5-吡唑啉酮进行了研究。校准曲线显示出良好的线性，相关系数平方 > 0.995。加标回收率确定为 91.1-105.7%，相对日内和日间标准偏差范围分别为 2.58-6.71% 和 3.15-7.67%。所有 10 种单糖的检测限和定量限分别为 0.02 至 0.10 μg/mL 和 0.05 至 0.25 μg/mL。使用这种方法，包含多糖的单糖 分别为 15–7.67%。所有 10 种单糖的检测限和定量限分别为 0.02 至 0.10 μg/mL 和 0.05 至 0.25 μg/mL。使用这种方法，包含多糖的单糖 分别为 15–7.67%。所有 10 种单糖的检测限和定量限分别为 0.02 至 0.10 μg/mL 和 0.05 至 0.25 μg/mL。使用这种方法，包含多糖的单糖易碎的鳕鱼被确定为阿拉伯糖、半乳糖和葡萄糖。