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Biochemical resistance mechanisms against chlorpyrifos, imidacloprid and lambda-cyhalothrin in Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae)
Crop Protection ( IF 2.8 ) Pub Date : 2021-02-03 , DOI: 10.1016/j.cropro.2021.105568
Rizwan Ahmed , Shoaib Freed

Rhynchophorus ferrugineus is the serious insect pest of palm groves. Chemical control against this species is an important constituent for developing an IPM strategy. But repeated use of the same class of synthetic chemicals to control a pest can cause resistance. The research was conducted to evaluate resistance in R. ferrugineus against commonly used synthetic insecticides (chlorpyrifos, imidacloprid and lambda-cyhalothrin) with intense selection pressure for seven successive generations under laboratory conditions. Further, biochemical assays were performed after selection with chlorpyrifos (Chlor-SEL), imidacloprid (Imida-SEL), and lambda-cyhalothrin (Lam-SEL) from G1 to G7 to estimate possible resistance mechanism against tested insecticides. The Imida-SEL, Chlor-SEL and Lam-SEL showed very high resistance 1883-fold, 488-fold and 112-fold, respectively at G7 compared to the susceptible population. Biochemical results showed that the highest activity of AChE (71.43 μmol/min mg protein) was majorly contributed in imidacloprid resistance, EST in chlorpyrifos resistance (87.18 μmol/min mg protein) and GST (83.93 μmol/min mg protein) in lambda-cyhalothrin resistance. The outcomes of the present research propose that the increased levels of enzymes have their possible role for the resistance development in R. ferrugineus and their suppression can aid in the breaking of resistance. The rotation or replacement of chlorpyrifos, and imidacloprid with lambda-cyhalothrin can be integrated for the management of R. ferrugineus.



中文翻译:

Rhynchophorus ferrugineus(Olivier)(鞘翅目:Curculionidae)中毒死rif,吡虫啉和氟氯氰菊酯的生化抗性机制

Ferrugineus Rhynchophorus ferrugineus是棕榈树的严重害虫。对这种物种的化学控制是制定IPM策略的重要组成部分。但是重复使用相同种类的合成化学物质来控制害虫可能会产生抗药性。这项研究是在实验室条件下连续七个世代评估R. ferrugineus对常用合成杀虫剂(毒死rif,吡虫啉和λ-氯氟氰菊酯)的抵抗力。此外,用毒死rif(Chlor-SEL),吡虫啉(Imida-SEL)和λ-氟氯氰菊酯(Lam-SEL)从G 1到G 7进行选择后,进行生化分析。估计可能对测试的杀虫剂有抗药性。与易感人群相比,Imida-SEL,Chlor-SEL和Lam-SEL在G 7分别显示出非常高的抗性,分别为1883-488、488-112和112-fold 。生化结果表明,AChE的最高活性(71.43μmol/ min mg蛋白)主要是对吡虫啉的抗性,EST对毒死(的抗性(87.18μmol/ min mg的蛋白)和GST(83.93μmol/ min mg的蛋白)在氟氯氰菊酯中具有重要作用。抵抗性。本研究的结果表明,增加的酶水平可能对R. ferrugineus的抗药性产生影响抑制它们可以帮助抵抗力量的破坏。可以将轮虫或毒死,和吡虫啉用lambda-cyhalothrin替换或替换,以用于治疗R. ferrugineus

更新日期:2021-02-07
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