Plant height (PH) determines the morphology and seed yield of soybean, so it is an important breeding target, which is controlled by multiple genes and affected by plant density. In this research, it was used about a four-way recombinant inbred lines (FW-RIL) with 144 families constructed by double cross (Kenfeng 14 × Kenfeng 15) × (Heinong 48 × Kenfeng 19) as experimental materials, with the purpose to map QTL/QTN associated with PH under densities of 2.2×10⁵ plant/ha (D1) and 3×10⁵ plant/ha (D2) in five environments. The results showed that response of PH to densities varied in accordance to genotypes among environments. A total of 26 QTLs and 13 QTNs were identified specifically in D1; 20 QTLs and 21 QTNs were identified specifically in D2. Nine QTLs and one QTN were discovered commonly in two densities. Fifteen QTLs and 9 QTNs were repeatedly detected by multiple statistical methods, densities, or environments, which could be considered stable. Eighteen QTLs were detected, as well as 7 QTNs underlying responses of PH to density increment. Six QTNs, co-located in the interval of QTL, were detected in more than two environments or methods with a longer genome length over 3000 kb. Based on gene ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, five genes were predicted as candidates, which were likely to be involved in growth and development of PH. The results will help elucidate the genetic basis and improve molecular assistant selection of PH.

株高（PH）决定大豆的形态和种子产量，因此是重要的育种目标，受多个基因控制并受植株密度影响。本研究以双交（垦丰14×垦丰15）×（黑农48×垦丰19）构建的144个家系的四向重组自交系（FW-RIL）为实验材料，旨在绘制了五种环境中2.2×10 ⁵植物/公顷(D1)和3×10 ⁵植物/公顷(D2)密度下与PH相关的QTL/QTN 。结果表明，PH 对密度的响应根据环境中的基因型而变化。D1共特异鉴定出26个QTL和13个QTN；D2 中特异鉴定出 20 个 QTL 和 21 个 QTN。两种密度中常见 9 个 QTL 和 1 个 QTN。通过多种统计方法、密度或环境重复检测到15个QTL和9个QTN，可以认为是稳定的。检测到 18 个 QTL，以及 7 个 QTN，这些 QTN 是 PH 对密度增加的反应的基础。在两种以上基因组长度超过 3000 kb 的环境或方法中检测到 6 个 QTN，共位于 QTL 区间。基于基因本体（GO）富集分析和京都基因与基因组百科全书（KEGG）分析，预测了5个可能参与PH生长发育的候选基因。研究结果将有助于阐明PH的遗传基础并改进PH的分子辅助选择。