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Foliar Spray of Silicon Enhances Resistance against Pyricularia oryzae by Triggering Phytoalexin Responds in Aerobic Rice
European Journal of Plant Pathology ( IF 1.8 ) Pub Date : 2021-02-03 , DOI: 10.1007/s10658-020-02197-1
Lee Chuen Ng , Z. Nura Adila , Elham M. Shahrul Hafiz , A. Aziz

Rice blast (caused by Pyricularia oryzae) is the most devastative disease threatening sustainability rice production. The role of silicon as a foliar application in biochemical defence involvement towards P. oryzae infection in aerobic rice remains unclear. The goal of this study was to explore the effectiveness of silicon (Si) as a foliar spray in triggering phytoalexin synthesis to increase resistance against P. oryzae infection on two aerobic rice cultivars MR219-4 (partially resistant to rice blast) and MARDI Aerob 1 (resistant). Three Si sources: calcium silicate, silica oxide and sodium metasilicate were tested at 3, 6, and 9 mg/L against P. oryzae infection. Foliar application of calcium silicate at 9 mg/L significantly reduced rice blast disease severity to 0.11% (MARDI Aerob 1) and 3.30% (MR219-4). In vitro screenings of phytoalexins (sakuranetin and momilactone A) against P. oryzae revealed that sakuranetin is more prominent to inhibit P. oryzae in both solid and liquid media. The expression of sakuranetin was further quantified from Si-mediated rice seedlings after challenge inoculated with P. oryzae. High-Performance Liquid Chromatography (HPLC) revealed that sakuranetin in both Si-mediated rice seedlings inoculated with P. oryzae significantly increased at 2nd DAI with 0.13 mg/mL in MARDI Aerob 1 and 0.48 mg/mL in MR219-4 compared with control (without Si foliar application). These results emphasized calcium silicate as foliar spray augmented resistance to P. oryzae infection by prolonging sakuranetin accumulation as one of the mechanisms that strengthening the rice plant health besides physiological barrier mechanism in the aerobic rice.



中文翻译:

硅叶面喷雾通过引发好氧性水稻中的植物抗毒素反应增强稻瘟病菌的抗性

稻瘟病(由稻瘟病菌引起)是最具破坏性的疾病,威胁到稻米的可持续生产。尚不清楚硅作为叶面应用在好氧水稻中对稻瘟病菌感染的生化防御作用中的作用。这项研究的目的是探索硅(Si)作为叶面喷雾剂在触发植物抗毒素合成以增加对两个需氧水稻品种MR219-4(部分抗稻瘟病)和MARDI Aerob 1的米曲霉感染的抵抗力方面的有效性。 (耐)。三硅来源:硅酸钙,二氧化硅和氧化钠偏硅酸物在3,6测试和9毫克/升对P.米曲霉感染。叶面喷施9 mg / L的硅酸钙可将稻瘟病的严重程度显着降低至0.11%(MARDI Aerob 1)和3.30%(MR219-4)。对米曲霉的植物抗毒素(sakuranetin和妈咪内酯A)的体外筛选显示,sakuranetin在固体和液体培养基中均能更有效地抑制米曲霉。在用稻瘟病菌接种后,从Si介导的水稻幼苗中进一步定量了sakuranetin的表达。高效液相色谱法(HPLC)显示,米黄假单胞菌接种硅介导的两种水稻幼苗中的sakuranetin与对照组相比(未施用硅叶),第二次DAI显着增加,MARDI Aerob 1中为0.13 mg / mL,MR219-4中为0.48 mg / mL。这些结果强调了叶面喷施硅酸钙通过延长樱红素的积累来增强对稻瘟病菌的抵抗力,这是除有氧水稻中的生理屏障机制外,增强稻米健康的机制之一。

更新日期:2021-02-03
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