Abstract

This study was conducted to evaluate the anti-diabetic and antioxidant effects of hydroalcoholic pomegranate peel extract (APE) in alloxan-induced diabetes rat models. We divided 60 rats into the following six equal groups (n = 10): Healthy control; diabetic control (100 mg/kg alloxan); sham + glibenclamide (10 mg/kg); diabetic + glibenclamide (10 mg/kg); sham + APE (200 mg/kg) and diabetic + APE (200 mg/kg). After 8 weeks, kidneys were taken out for biochemical and molecular studies. Following APE treatment, biochemical parameters including malondialdehyde (MDA), and glutathione (GSH), glutathione peroxidase (GPx), catalase (CAT), superoxide dismutase (SOD) significantly induced in the treated group as compared with the control group (p < 0.05). Also, gene expression of GPx (3-fold), CAT (2.6-fold), and SOD (1.5-fold) were increased as compared to controls (p < 0.05). Overall, our results indicated that pomegranate can be used as an antioxidant agent to reduce complications from diseases associated with oxidative stress.

摘要

本研究旨在评估水醇石榴皮提取物 (APE) 在四氧嘧啶诱导的糖尿病大鼠模型中的抗糖尿病和抗氧化作用。我们将 60 只大鼠分为以下 6 个相等组（n  = 10）： 健康对照组；糖尿病控制（100 mg/kg 四氧嘧啶）；假手术 + 格列本脲 (10 mg/kg)；糖尿病+格列本脲（10毫克/公斤）；假手术 + APE (200 mg/kg) 和糖尿病 + APE (200 mg/kg)。8周后，取出肾脏进行生化和分子研究。APE治疗后，与对照组相比，治疗组的生化参数包括丙二醛（MDA）、谷胱甘肽（GSH）、谷胱甘肽过氧化物酶（GPx）、过氧化氢酶（CAT）、超氧化物歧化酶（SOD）显着升高（p < 0.05  ） ）。此外，与对照相比，GPx（3 倍）、CAT（2.6 倍）和SOD （1.5 倍）的基因表达增加（ p  < 0.05）。总的来说，我们的结果表明石榴可以用作抗氧化剂，以减少与氧化应激相关疾病的并发症。