The expanding phylogenetic tree of trypanosomatid flagellates (Kinetoplastea: Trypanosomatidae) contains a long-known and phylogenetically well-supported species-rich lineage that was provisionally named as the ‘jaculum’ clade. Its members were found in representatives of several unrelated families of heteropteran bugs captured in South and Central America, Europe, Africa, and Asia. However, this group resisted introduction into the culture, a needed prerequisite for its proper characterization. Here we describe four new cultivable species, which parasitize various parts of their hosts’ intestine, including the thoracic and abdominal part of the midgut, hindgut, and Malpighian tubules. Morphologically, the cultured flagellates vary from relatively short stumpy promastigotes to long slender leptomonad cells. Some species form straphangers (cyst-like amastigotes) both in vivo and in vitro, initially attached to the basal part of the flagellum of the mother cell, from which they subsequently detach. To formally classify this enigmatic monophyletic cosmopolitan clade, we erected Obscuromonas gen. nov., including five species: O. modryi sp. nov. (isolated from the true bug host species Riptortus linearis captured in the Philippines), O. volfi sp. nov. (from Catorhintha selector, Curaçao), O. eliasi sp. nov. (from Graptostethus servus, Papua New Guinea), O. oborniki sp. nov. (from Aspilocoryphus unimaculatus, Madagascar), and O. jaculum comb. nov. (from Nepa cinerea, France). Obscuromonas along with the genus Blastocrithidia belongs to the newly established Blastocrithidiinae subfam. nov.

不断扩大的锥虫鞭毛虫（动植物：锥虫科）的系统发育树包含一个长期存在且系统发育良好的物种丰富的谱系，该谱系被临时命名为“ Jaculum”。进化枝。在南美洲和中美洲，欧洲，非洲和亚洲捕获的几个不相关的异翅类昆虫家族的代表中发现了它的成员。但是，该小组拒绝引入文化，这是对其进行正确表征的必要先决条件。在这里，我们描述了四个新的可培养物种，它们寄生了寄主肠道的各个部分，包括中肠，后肠和马尔皮基小管的胸部和腹部。从形态上讲，培养的鞭毛从相对短的树桩前鞭毛体到长而纤细的钩端螺旋体细胞不等。一些物种在体内和体外均会形成钩挂物（囊肿状变形虫）最初附着于母细胞鞭毛的基部，随后从中脱离。为了正式分类这个神秘的单系世界性进化枝，我们架设了Obscuromonas gen。十一月，包括五种：O. modryi sp.。十一月 （与从菲律宾捕获的真正的虫子宿主物种Riptortus linearis分离），O。volfi sp.。十一月 （来自Catorhintha选择者Curaçao），O。eliasi sp.。十一月 （来自巴布亚新几内亚的Graptostethus servus），O。oborniki sp.。十一月 （来自马达加斯加的Aspilocoryphus unimaculatus）和O. jaculum梳子。十一月 （从法国的Nepa cinerea）。Obscuromonas与Blastocrithidia属一起属于新近建立的Blastocrithidiinae亚家族。十一月