The production of reproducible batches of liposomes suitable for parenteral administration is a time-consuming, multi-step process requiring extensive workable area. Microfluidic Hydrodynamic Focusing (MHF) chips allow one-pot synthesis of injectable size liposomes, although the use of low height-to-width aspect ratio (AR) microreactors limits the production rates.

Herein, aiming at scaling up liposome production, while avoiding lithographic methods and clean room facilities, easy-fabrication glass MHF large channel (MHF-LC) chips of AR = 1, 3 with T-shaped and coaxial injection geometries are demonstrated. Narrowly distributed unilamellar nanoliposomes (unprecedented minimum average diameters 85 nm and polydispersity index of 0.13) with the formulation of a known pharmaceutical product (i.e., DOXIL®(CAELYX®)) are synthesized at production rates 15–20 times larger than T-MHF chips. The dependence of liposome size on the Reynolds numbers (in the range of 5–50) in the coaxial configuration is discussed as due to viscosity-induced mixing dynamics at the water–ethanol interface.

适于肠胃外给药的可重制脂质体批次的生产是耗时的，多步骤的过程，需要广阔的工作区域。尽管使用低高宽比（AR）的微反应器会限制生产率，但微流体流体动力聚焦（MHF）芯片可以一锅合成大小可注射的脂质体。

在本文中，为了扩大脂质体的生产，同时避免了光刻方法和无尘室设施，展示了AR = 1、3的T形和同轴注射几何形状的易于制造的玻璃MHF大通道（MHF-LC）芯片。具有已知药物（例如DOXIL®（CAELYX®））配方的窄分布单层纳米脂质体（空前的最小平均直径为85 nm，多分散指数为0.13）比T-MHF芯片大15-20倍。讨论了在同轴构型中脂质体大小对雷诺数（在5-50范围内）的依赖性，这是由于在水-乙醇界面处粘度引起的混合动力学。