Huanglongbing (HLB), caused by Candidatus Liberibacter asiaticus (CLas), is an important and threatening disease for the citru s industry in southeast Iran. In the present study, seasonal dynamic monitoring of CLas was conducted by absolute real-time PCR. Plant materials were obtained across 7 and 6 months from December 2014 to October 2015 from three parts of the canopy of two naturally infected citrus species, Mexican lime (Citrus aurantifolia) and sweet orange (C. sinensis) located in two distinct geographical regions, Sistan-o-Baluchestan and Kerman provinces, respectively. Results showed significant variation (α = 0.001) in the bacterial titers in both infected hosts. These notable changes have also been observed in all tree’s canopy parts and a particular part of tree canopy during sampling months, exhibited by sudden changes in bacterial titers. It was also found that in both locations maximum titers of HLB occurred in summer. The data obtained by correlation assessment suggests a significant relationship between temperature and pathogen population variations that probably in warmer months correlated with the sap flow rate changes in the host, the bacterial titer was increased. In this study, no consistent pattern of the disease agent distribution was found between two different plant hosts. The results of this research on the quantification of CLas in the host plant can be a great help in making proper decisions for disease management by providing the possibility of early detection and rapid removal of inoculum sources.

黄龙病（HLB），引起暂定Liberibacter胭脂（CLAS），是为citru的行业在伊朗东南部的重要，并威胁疾病。在本研究中，通过绝对实时PCR对CLA进行季节性动态监测。从2014年12月至2015年10月的7到6个月中，从两种自然感染的柑橘树种的三部分冠层中获得了植物材料，这两种物种分别是墨西哥石灰（Citrus aurantifolia）和甜橙（C. sinensis）。）分别位于两个不同的地理区域（锡斯坦-o-巴鲁什斯坦和克尔曼省）。结果表明，两种感染宿主的细菌滴度均存在显着差异（α= 0.001）。在采样月份的所有树木冠层部分和树木冠层的特定部分中也观察到了这些显着变化，表现为细菌滴度的突然变化。还发现在两个位置，HLB的最大效价在夏季发生。通过相关性评估获得的数据表明，温度与病原体种群变化之间存在显着的关系，这可能在温暖的月份与宿主液流速率的变化相关，细菌滴度增加。在这项研究中，在两个不同的植物宿主之间没有发现一致的病原菌分布模式。