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Ancestral role of TNF-R pathway in cell differentiation in the basal metazoan Hydra
Journal of Cell Science ( IF 4 ) Pub Date : 2021-01-25 , DOI: 10.1242/jcs.255422
Mona Steichele 1 , Lara S Sauermann 1 , Ann-Christine König 1 , Stefanie Hauck 1 , Angelika Böttger 1
Affiliation  

Mona Steichele, Lara S. Sauermann, Ann-Christine König, Stefanie Hauck, and Angelika Böttger

Tumour necrosis factor receptors (TNF-Rs) and their ligands, tumour necrosis factors, are highly conserved proteins described in all metazoan phyla. They function as inducers of extrinsic apoptotic signalling and facilitate inflammation, differentiation and cell survival. TNF-Rs use distinct adaptor molecules to activate signalling cascades. Fas-associated protein with death domain (FADD) family adaptors often mediate apoptosis, and TNF-R-associated factor (TRAF) family adaptors mediate cell differentiation and inflammation. Most of these pathway components are conserved in cnidarians, and, here, we investigated the Hydra TNF-R. We report that it is related to the ectodysplasin receptor, which is involved in epithelial cell differentiation in mammals. In Hydra, it is localised in epithelial cells with incorporated nematocytes in tentacles and body column, indicating a similar function. Further experiments suggest that it interacts with the Hydra homologue of a TRAF adaptor, but not with FADD proteins. Hydra FADD proteins colocalised with Hydra caspases in death effector filaments and recruited caspases, suggesting that they are part of an apoptotic signalling pathway. Regulating epithelial cell differentiation via TRAF adaptors therefore seems to be an ancient function of TNF-Rs, whereas FADD–caspase interactions may be part of a separate apoptotic pathway.



中文翻译:

TNF-R 通路在基底后生动物九头蛇细胞分化中的祖先作用

Mona Steichele、Lara S. Sauermann、Ann-Christine König、Stefanie Hauck 和 Angelika Böttger

肿瘤坏死因子受体 (TNF-R) 及其配体肿瘤坏死因子是在所有后生动物门中描述的高度保守的蛋白质。它们作为外在凋亡信号传导的诱导剂起作用并促进炎症、分化和细胞存活。TNF-Rs 使用不同的接头分子来激活信号级联。Fas 相关蛋白与死亡域 (FADD) 家族适配器通常介导细胞凋亡,而 TNF-R 相关因子 (TRAF) 家族适配器介导细胞分化和炎症。大多数这些通路成分在刺胞动物中是保守的,在这里,我们研究了九头蛇TNF-R。我们报告说它与 ectodysplasin 受体有关,后者参与哺乳动物的上皮细胞分化。在九头蛇,它位于上皮细胞中,在触手和体柱中掺入了刺细胞,表明具有类似的功能。进一步的实验表明它与TRAF 接头的Hydra同源物相互作用,但不与 FADD 蛋白相互作用。Hydra FADD 蛋白与死亡效应丝中的Hydra半胱天冬酶共定位并募集半胱天冬酶,表明它们是凋亡信号通路的一部分。因此,通过 TRAF 接头调节上皮细胞分化似乎是 TNF-R 的古老功能,而 FADD-半胱天冬酶相互作用可能是单独的凋亡途径的一部分。

更新日期:2021-02-01
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