The budding yeast phosphatase Cdc14 has a central role in mitotic exit and cytokinesis. Puzzlingly, a uniform picture for the three human CDC14 paralogues CDC14A, CDC14B and CDC14C in cell cycle control has not emerged to date. Redundant functions between the three CDC14 phosphatases could explain this unclear picture. To address the possibility of redundancy, we tested expression of CDC14 and analysed cell cycle progression of cells with single and double deletions in CDC14 genes. Our data suggest that CDC14C is not expressed in human RPE1 cells, excluding a function in this cell line. Single- and double-knockouts (KO) of CDC14A and CDC14B in RPE1 cells indicate that both phosphatases are not important for the timing of mitotic phases, cytokinesis and cell proliferation. However, cycling CDC14A KO and CDC14B KO cells show altered ciliogenesis compared to wild-type cells. The cilia of cycling CDC14A KO cells are longer, whereas CDC14B KO cilia are more frequent and disassemble faster. In conclusion, this study demonstrates that the cell cycle functions of CDC14 proteins are not conserved between yeast and human cells.

出芽酵母磷酸酶 Cdc14 在有丝分裂退出和胞质分裂中具有核心作用。令人费解的是，迄今为止尚未出现三种人类 CDC14 旁系同源物 CDC14A、CDC14B 和 CDC14C 在细胞周期控制中的统一图景。三种 CDC14 磷酸酶之间的冗余功能可以解释这种不清楚的情况。为了解决冗余的可能性，我们测试了CDC14的表达并分析了CDC14基因中具有单缺失和双缺失的细胞的细胞周期进展。我们的数据表明CDC14C在人类 RPE1 细胞中不表达，不包括该细胞系中的功能。CDC14A和CDC14B的单敲除和双敲除 (KO)在 RPE1 细胞中表明两种磷酸酶对于有丝分裂期、胞质分裂和细胞增殖的时间并不重要。然而，与野生型细胞相比，循环CDC14A KO 和CDC14B KO 细胞显示纤毛发生改变。循环CDC14A KO 细胞的纤毛更长，而CDC14B KO 纤毛更频繁且分解更快。总之，这项研究表明 CDC14 蛋白的细胞周期功能在酵母和人类细胞之间不是保守的。