Neochloris minuta and Neochloris alveolaris grown in nitrogen-rich (+ N) and nitrogen-depleted (-N) media were tested for their heavy metal maximum biosorption capacities (q_max) and adsorption percent efficiencies (R%). By removing nitrogen from the growth media, both algal species showed an increase in their lipid content and a decrease in their protein content. Langmuir and Freundlich adsorption isotherms were used to determine the q_max and adsorption efficiencies of the + N and −N algae in the recovery of Pb²⁺, Cd²⁺, Zn²⁺, Cu²⁺, and Ni²⁺. When comparing the two types of algae, N. alveolaris showed the highest adsorption capacities for all five metals either in + N or -N media. The maximum adsorption efficiency percentage of the lowest concentration metal ions for N. alveolaris was 87.10% for Pb²⁺, 64.98% for Cd²⁺, 59.50% for Zn²⁺, 60.08% for Cu²⁺, and 50.61% for Ni²⁺. In both algae, nitrogen depletion (-N) caused an increase in the q_max values for Zn²⁺ and Cu²⁺. Additionally, the q_max of N. minuta for Cd²⁺, Zn²⁺, Cu²⁺ and Ni²⁺ increased by the nitrogen depletion demonstrating that the treatment can be applied to improve the biosorption capacity of a particular alga for multiple heavy metals. The biosorption capacity for these algae for heavy metals was also discussed in terms of their biomass compositions and the type of hard or soft metal acid based on the Pearson theory of Hard and Soft, Acid and Bases (HSAB).

测试了在富氮（+ N）和贫氮（-N）培养基中生长的小球藻（Neochloris minuta）和小球藻（Neochloris alveolaris）的重金属最大生物吸附量（q _max）和吸附百分效率（R％）。通过从生长培养基中除去氮，两种藻类均显示出其脂质含量的增加和其蛋白质含量的减少。用Langmuir和Freundlich吸附等温线确定+ N和-N藻类在回收Pb 2 ⁺，Cd 2 ⁺，Zn 2 ⁺，Cu ²⁺和Ni ^2+时的q _max和吸附效率。比较两种藻类时，肺泡猪笼草在+ N或-N介质中，对五种金属均显示出最高的吸附能力。最低浓度的金属离子对肺泡线虫的最大吸附效率百分比分别为Pb ²⁺为87.10％，Cd ²⁺为64.98 ％，Zn ²⁺为59.50 ％，Cu ²⁺为60.08 ％，Ni ²为50.61％⁺。在两种藻类中，氮耗竭（-N）导致Zn ²⁺和Cu ²⁺的q _max值增加。另外，将q_最大的N.小粒镉²⁺，锌²⁺，铜²⁺和Ni ²⁺氮消耗的增加表明该处理可用于提高特定藻类对多种重金属的生物吸附能力。根据硬质和软质，酸和碱的皮尔逊理论（HSAB），还根据藻类的生物量组成以及硬质或软质金属酸的类型，讨论了这些藻类对重金属的生物吸附能力。