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In Vitro Methods to Model Cardiac Mechanobiology in Health and Disease
Tissue Engineering, Part C: Methods ( IF 3 ) Pub Date : 2021-03-15 , DOI: 10.1089/ten.tec.2020.0342
Ignasi Jorba 1, 2 , Dylan Mostert 1, 2 , Leon H L Hermans 1, 2 , Atze van der Pol 1, 2 , Nicholas A Kurniawan 1, 2 , Carlijn V C Bouten 1, 2
Affiliation  

In vitro cardiac modeling has taken great strides in the past decade. While most cell and engineered tissue models have focused on cell and tissue contractile function as readouts, mechanobiological cues from the cell environment that affect this function, such as matrix stiffness or organization, are less well explored. In this study, we review two-dimensional (2D) and three-dimensional (3D) models of cardiac function that allow for systematic manipulation or precise control of mechanobiological cues under simulated (patho)physiological conditions while acquiring multiple readouts of cell and tissue function. We summarize the cell types used in these models and highlight the importance of linking 2D and 3D models to address the multiscale organization and mechanical behavior. Finally, we provide directions on how to advance in vitro modeling for cardiac mechanobiology using next generation hydrogels that mimic mechanical and structural environmental features at different length scales and diseased cell types, along with the development of new tissue fabrication and readout techniques.

中文翻译:

健康和疾病中心脏机械生物学模型的体外方法

体外心脏模型在过去十年中取得了长足的进步。虽然大多数细胞和工程组织模型都将重点放在细胞和组织收缩功能上作为读数,但影响该功能的细胞环境的机械生物学线索(例如基质刚度或组织)却很少被探索。在这项研究中,我们回顾了心脏功能的二维 (2D) 和三维 (3D) 模型,这些模型允许在模拟(病理)生理条件下系统地操纵或精确控制机械生物学线索,同时获取细胞和组织功能的多个读数。我们总结了这些模型中使用的细胞类型,并强调了连接 2D 和 3D 模型以解决多尺度组织和机械行为的重要性。最后,我们提供了如何使用下一代水凝胶推进心脏机械生物学的体外建模的方向,该水凝胶模拟不同长度尺度和患病细胞类型的机械和结构环境特征,以及新的组织制造和读出技术的发展
更新日期:2021-03-18
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