Florida red tides have become more common and persistent in and around the Gulf of Mexico. When in bloom, red tides can produce brevetoxins in high concentrations, leading to human exposures primarily through contaminated food and ocean spray. The research described here includes adapting and validating a commercial brevetoxin water test kit for human plasma testing. Pooled plasma was fortified with a model brevetoxin, brevetoxin 3, at concentrations from 0.00500 to 3.00 ng/mL to generate calibration curves and quality control samples. The quantitative detection range was determined to be 0.0400–2.00 ng/mL brevetoxin 3 equivalents with inter- and intraday accuracies ranging from 94.0% to 109% and relative standard deviations <20%, which is within the US Food and Drug Administration guidelines for receptor-binding assays. Additionally, cross-reactivity was tested using 4 of the 10 known brevetoxins and 12 paralytic shellfish toxins. The cross-reactivity varied from 0.173% to 144% for the commercially available brevetoxin standards and 0% for the commercially available paralytic shellfish toxin standards. Fifty individual unexposed human plasma samples were measured to determine the limit of detection and endogenous interferences to the test. The validated method was used to test 31 plasma samples collected from humans potentially exposed to brevetoxins, detecting 11 positives. This method has been proven useful to measure human exposure to brevetoxins and can be applied to future exposure events.

中文翻译：

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ELISA检测人血浆中的Brevetoxin

佛罗里达赤潮在墨西哥湾及其周边地区变得更加普遍和持久。在开花时，赤潮会产生高浓度的短尾毒素，主要通过受污染的食物和海洋喷雾导致人类暴露。此处描述的研究包括调整和验证用于人体血浆检测的商业短尾毒素水检测试剂盒。用浓度为 0.00500 至 3.00 ng/mL 的模型短毒素（短毒素 3）强化混合血浆，以生成校准曲线和质量控制样品。定量检测范围确定为 0.0400–2.00 ng/mL 短尾毒素 3 当量，日间和日内准确度范围为 94.0% 至 109%，相对标准偏差 <20%，符合美国食品和药物管理局关于受体结合测定。此外，使用 10 种已知短尾毒素中的 4 种和 12 种麻痹性贝类毒素测试了交叉反应性。市售的短尾鱼毒素标准品的交叉反应性从 0.173% 到 144% 不等，而市售的麻痹性贝类毒素标准品的交叉反应性为 0%。测量了 50 个未暴露的人血浆样本以确定检测限和对测试的内源性干扰。经验证的方法用于测试从可能暴露于短尾毒素的人类身上采集的 31 份血浆样本，检测出 11 份阳性结果。该方法已被证明可用于测量人类对短尾毒素的暴露，并可应用于未来的暴露事件。173% 到 144% 用于市售的短尾鱼毒素标准品和 0% 用于市售的麻痹性贝类毒素标准品。测量了 50 个未暴露的人血浆样本以确定检测限和对测试的内源性干扰。经验证的方法用于测试从可能暴露于短尾毒素的人类身上采集的 31 份血浆样本，检测出 11 份阳性结果。该方法已被证明可用于测量人类对短尾毒素的暴露，并可应用于未来的暴露事件。173% 至 144% 用于市售的短尾鱼毒素标准品和 0% 用于市售的麻痹性贝类毒素标准品。测量了 50 个未暴露的人血浆样本以确定检测限和对测试的内源性干扰。经验证的方法用于测试从可能暴露于短尾毒素的人类身上采集的 31 份血浆样本，检测出 11 份阳性结果。该方法已被证明可用于测量人类对短尾毒素的暴露，并可应用于未来的暴露事件。经验证的方法用于测试从可能暴露于短尾毒素的人类身上采集的 31 份血浆样本，检测出 11 份阳性结果。该方法已被证明可用于测量人类对短尾毒素的暴露，并可应用于未来的暴露事件。经验证的方法用于测试从可能暴露于短尾毒素的人类身上采集的 31 份血浆样本，检测出 11 份阳性结果。该方法已被证明可用于测量人类对短尾毒素的暴露，并可应用于未来的暴露事件。