Conservation tillage incorporating no-till (NT) is often recommended to reduce soil organic carbon (SOC) mineralization and sequester SOC, thus mitigating carbon dioxide production. The rates of SOC accumulation and mineralization are significantly affected by the time since the introduction of NT, however, there is currently poor understanding of exactly how rates change over time under long-term NT. Thus, an 11-year field experiment, a 60-day laboratory incubation, and a meta-analysis were used to better understand the effects of NT, experiment duration, and their interaction on SOC accumulation and mineralization. Our field experiment showed that, compared with plow tillage (PT), NT had a higher proportion of more stable SOC fractions and significantly increased SOC concentration by 12.6 % in the 5^th year of the field experiment, and 52.2 % in the 11^th year. A significant interaction between tillage and experiment duration was observed for SOC mineralization using laboratory incubation. NT decreased bulk SOC mineralization by 20.1 % in the 5^th year of the field experiment and by 44.8 % in the 11^th year when compared with PT. This was associated with an increase in SOC concentration and a decrease in SOC mineralization from macro-aggregates (>2 mm) over time and thus the accumulation of SOC was attributed to the physical protection of SOC within macro-aggregates. Meta-analysis confirmed these results and found that, compared with PT, NT significantly decreased SOC mineralization by ∼11 % when experiment duration was ≤8 years and by ∼27 % when it was 8–16 years (P < 0.05). This indicated that SOC mineralization was reduced in NT to an increasing degree as experiment duration increased and additional SOC could be sequestered with an experiment duration of up to 16 years.

通常建议采用免耕（NT）的保护性耕作，以减少土壤有机碳（SOC）的矿化和隔离SOC，从而减少二氧化碳的产生。自从引入NT以来，SOC的累积和矿化速率受时间的影响很大，但是，目前对于长期NT下速率随时间的确切变化了解甚少。因此，进行了为期11年的野外实验，60天的实验室孵化和荟萃分析，以更好地了解NT的影响，实验持续时间及其相互作用对SOC积累和矿化的影响。我们的田间试验表明，与犁耕作（PT）相比，NT有更稳定的SOC的馏分的比例较高和12.6％，在5显著增加SOC浓度^次田间试验，并在11 52.2％的年^日一年。使用实验室培养，观察到SOC矿化的耕作与实验持续时间之间存在显着的相互作用。NT 20.1％在5体积减小SOC矿化^个田间试验的和年中的11 44.8％^第与PT比较的年份。随着时间的推移，这与SOC浓度的增加和大型聚集体中SOC矿化的减少（> 2 mm）有关，因此SOC的累积归因于大型聚集体中SOC的物理保护。荟萃分析证实了这些结果，并发现，与PT相比，当实验持续时间≤8年时，NT显着降低SOC矿化约11％，而当实验8至16年时，则使SOC矿化降低约27％（P <0.05）。这表明随着实验持续时间的增加，NT中的SOC矿化程度降低，并且长达16年的实验持续时间可以隔离更多的SOC。