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Molecular identification and temporal genetic differentiation of Cyprinus carpio (Cypriniformes: Cyprinidae) eggs attached on artificial fish nests
Conservation Genetics Resources ( IF 1.1 ) Pub Date : 2021-01-30 , DOI: 10.1007/s12686-021-01189-5
Weitao Chen , Shuli Zhu , Xinhui Li , Yuefei Li

Introduction

Reproductive isolation by time can occur in fish populations composed of individuals with different reproductive schedules. Most studies have tested this phenomenon using mature fish rather than their offspring, which may mask signals of population relationships.

Methods

We directly sampled fish eggs from artificial fish nests at four different time instances and subsequently fed the eggs to fish larvae in an indoor laboratory. Fish larvae, of each timeline, were randomly selected for species identification using the mitochondrial cytochrome c oxidase I (COI) gene and performed population analyses utilizing the concatenated sequences of COI gene and the mitochondrial control region (D-loop).

Results

Neighbor-joining tree and Automatic Barcode Gap Discovery (ABGD) using COI sequences showed that all analyzed larvae can be assigned to the common carp (Cyprinus carpio). Significant differentiation among four demes inferred from the evidence of the concatenated sequences indicated temporal structuring in C. carpio demes within a reproductive season. Haplotype networks and a Bayesian method in BAPS also detected signals of population differentiation among the four demes.

Conclusion

The pattern observed in C. carpio demes revealed the reproductive strategy of the species and provided knowledge allowing establishment of conservation units.



中文翻译:

附着在人工鱼巢上的鲤鱼卵的分子鉴定和时间遗传分化

介绍

按时间进行生殖隔离可能发生在由具有不同生殖计划的个体组成的鱼类种群中。大多数研究使用成熟的鱼类而不是其后代来测试这种现象,这可能掩盖了种群关系的信号。

方法

我们在四个不同的时间点直接从人工鱼巢中取样鱼卵,然后在室内实验室将卵喂入鱼幼体。使用线粒体细胞色素C氧化酶I(COI)基因随机选择每个时间轴上的鱼幼虫进行物种鉴定,并使用COI基因和线粒体控制区的串联序列(D-loop)进行种群分析。

结果

邻居加入树和使用COI序列的自动条形码间隙发现(ABGD)表明,所有分析的幼虫都可以分配给鲤鱼(Cyprinus carpio)。从级联序列的证据中推断出的四个特征之间的显着差异表明,在繁殖季节内,鲤鱼角的特征是时间结构。BAPS中的单倍型网络和贝叶斯方法也检测到四个居间之间的种群分化信号。

结论

鲤鱼角膜藻中观察到的模式揭示了该物种的繁殖策略,并提供了建立保护单位的知识。

更新日期:2021-01-31
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