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Differentially Expressed Circular RNA Profile in an Intracranial Aneurysm Group Compared with a Healthy Control Group
Disease Markers ( IF 3.464 ) Pub Date : 2021-01-29 , DOI: 10.1155/2021/8889569
Yonggang Ma 1, 2, 3 , Baoqing Zhang 4 , Dong Zhang 1, 2, 3 , Shuo Wang 1, 2, 3 , Maogui Li 1, 2, 3 , Jizong Zhao 1, 2, 3
Affiliation  

Objective. Intracranial aneurysm (IA) is a fatal disease owing to vascular rupture and subarachnoid hemorrhage. Much attention has been given to circular RNAs (circRNAs) because they may be potential biomarkers for many diseases, but their mechanism in the formation of IA remains unknown. Methods. circRNA expression profile analysis of blood samples was conducted between patients with IA and controls. Overall, 235 differentially expressed circRNAs were confirmed between IA patients and the control group. The reliability of the microarray results was demonstrated by quantitative real-time polymerase chain reaction (qRT-PCR). Results. Of 235 differentially expressed genes, 150 were upregulated, while the other 85 were downregulated. Five miRNAs matched to every differential expression of circRNAs, and related MREs were predicted. We performed gene ontology (GO) analysis to identify the functions of their targeted genes, with the terms “Homophilic cell adhesion via plasma membrane adhesion molecules” and “Positive regulation of cellular process” showing the highest fold enrichment. Conclusions. This study demonstrated the role of circRNA expression profiling in the formation of IA and revealed that the mTOR pathway can be a latent therapeutic strategy for IA.

中文翻译:

颅内动脉瘤组与健康对照组相比差异表达的环状 RNA 谱

客观。颅内动脉瘤 (IA) 是由于血管破裂和蛛网膜下腔出血而导致的致命疾病。环状 RNA (circRNA) 受到了很多关注,因为它们可能是许多疾病的潜在生物标志物,但它们在 IA 形成中的机制仍然未知。方法。在 IA 患者和对照组之间进行血液样本的 circRNA 表达谱分析。总体而言,在 IA 患者和对照组之间确认了 235 个差异表达的 circRNA。通过定量实时聚合酶链反应 (qRT-PCR) 证明了微阵列结果的可靠性。结果. 在 235 个差异表达基因中,150 个上调,而其他 85 个下调。5 个 miRNA 与 circRNA 的每个差异表达相匹配,并预测了相关的 MRE。我们进行了基因本体论 (GO) 分析以识别其靶向基因的功能,其中术语“通过质膜粘附分子的同源性细胞粘附”和“细胞过程的正向调节”显示出最高的倍数富集。结论。该研究证明了 circRNA 表达谱在 IA 形成中的作用,并揭示了 mTOR 途径可以成为 IA 的潜在治疗策略。
更新日期:2021-01-29
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