In this paper, we demonstrate a digital micromirror device (DMD) based optical microscopic apparatus for quantitative differential phase contrast (qDIC) imaging, coherent structured illumination microscopy (SIM), and dual-modality (scattering/fluorescent) imaging. For both the qDIC imaging and the coherent SIM, two sets of fringe patterns with orthogonal orientations and five phase-shifts for each orientation, are generated by a DMD and projected on a sample. A CCD camera records the generated images in a defocusing manner for qDIC and an in-focus manner for coherent SIM. Both quantitative phase images and super-resolved scattering/fluorescence images can be reconstructed from the recorded intensity images. Moreover, fluorescent imaging modality is integrated, providing specific biochemical structures of the sample once using fluorescent labeling.

在本文中，我们演示了一种基于数字微镜设备（DMD）的光学显微镜设备，用于定量微分相衬（qDIC）成像，相干结构照明显微镜（SIM）和双模态（散射/荧光）成像。对于qDIC成像和相干SIM，DMD会生成两组具有正交方向且每个方向有五个相移的条纹图案，并将其投影到样本上。CCD相机对于qDIC以散焦方式记录生成的图像，而对于相干SIM则以对焦方式记录生成的图像。定量相位图像和超分辨散射/荧光图像均可从记录的强度图像中重建。此外，集成了荧光成像方式，一旦使用荧光标记即可提供样品的特定生化结构。