Abstract

The molecular resistance mechanism of Aspergillus oryzae (A. oryzae) under arsenate (As(V)) stress was investigated by comparative transcriptome analysis. In total, 4911 differentially expressed genes (DEGs) of A. oryzae were detected under As(V) stress, including 2103 up-regulated genes and 2808 down-regulated genes. Genes induced by As(V) were involved in metal ion binding and transportation, redox homeostasis, efflux carrier, DNA repair, volatilization, etc. Moreover, eight genes were selected for qRT-PCR verification, and their trends were consistent with RNA-Seq analysis, which proved that RNA-Seq analysis was efficient and reliable. The results indicated that the extracellular and intracellular synergistic resistance mechanisms contributed to the improvement of A. oryzae resistance to As (V).

摘要

通过比较转录组分析研究了米曲霉（As。oryzae）在砷（As（V））胁迫下的分子抗性机理。总共有4911个米曲霉的差异表达基因（DEG）在As（V）胁迫下检测到，包括2103个上调基因和2808个下调基因。As（V）诱导的基因参与金属离子的结合和转运，氧化还原稳态，外排载体，DNA修复，挥发等。此外，选择了8个基因进行qRT-PCR验证，其趋势与RNA-Seq一致分析，证明RNA-Seq分析是有效和可靠的。结果表明，细胞外和细胞内协同抗性机制有助于提高米曲霉对As（V）的抗性。