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Production of a bacterial biosurfactant in an electrochemical environment as a prelude for in situ biosurfactant enhanced bio-electrokinetic remediation
Process Safety and Environmental Protection ( IF 7.8 ) Pub Date : 2021-01-28 , DOI: 10.1016/j.psep.2021.01.041
Brian Gidudu , Evans M. Nkhalambayausi Chirwa

The possibility of producing biosurfactants in an electrochemical environment was studied at three different currents of 0.5 A, 1 A and 1.5 A. The Pseudomonas aeruginosa strain was able to produce biosurfactant in all the three experiments with a yield of 36.25 ± 3.75 mg/mL, 22.5 ± 5 mg/mL, 6.25 ± 1.25 for 0.5 A, 1 A and 1.5 A respectively. A rhamnolipid biosurfactant with 7 mono-rhamnolipid and 4 di-rhamnolipid homologues was detected in all experiments. However, the survival and yield of the biosurfactant was affected by the intensity of the electric field applied. The highest electric field of 1.5 A led to total inactivation and elimination of the bacteria within the first 12 h while the bacteria survived to the end of the experiment in 96 h when 0.5 A and 1 A was applied.



中文翻译:

在电化学环境中生产细菌生物表面活性剂,作为原位生物表面活性剂增强的生物电动修复的序幕

研究了在0.5 A,1 A和1.5 A的三种不同电流下在电化学环境中生产生物表面活性剂的可能性。铜绿假单胞菌菌株在所有三个实验中均能够生产生物表面活性剂,产量为36.25±3.75 mg / mL, 0.5 A,1 A和1.5 A分别为22.5±5 mg / mL,6.25±1.25。在所有实验中均检测到具有7个单鼠李糖脂和4个二鼠李糖脂同系物的鼠李糖脂生物表面活性剂。但是,生物表面活性剂的存活率和产率受所施加电场强度的影响。1.5 A的最高电场导致在最初的12 h内细菌完全失活和消除,而使用0.5 A和1 A的细菌在96 h内存活到实验结束。

更新日期:2021-01-29
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