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Genetic variation and mating type distribution of Rhynchosporium commune in Turkey
Physiological and Molecular Plant Pathology ( IF 2.7 ) Pub Date : 2021-01-29 , DOI: 10.1016/j.pmpp.2021.101614
Arzu Çelik Oğuz , Fatih Ölmez , Aziz Karakaya , Mohammad Reza Azamparsa

Rhynchosporium commune is the causal agent of barley scald disease and is an important foliar disease of barley worldwide. During 2012, 2013, and 2014, Rhynchosporium commune single-spore isolates were collected from different parts of Turkey, and from those, 60 isolates were selected. The mating type distribution and prevalence of these selected isolates were determined. Twenty-one of the 60 single-spore isolates were found to be MAT1-1 type and 39 isolates were MAT1-2 type χ2 (1:1) types = 5.40 (P = 0.02). The mating type ratio showed that R. commune populations may have predominantly asexual reproduction in Turkey. To determine the genetic diversity of R. commune isolates, 5 single-spore isolates from different regions of Turkey were tested with 72 ISSR primers. Eleven primers were found to be polymorphic. UBC 826, UBC 861, UBC 886, UBC 887, UBC 890, UBC 848, UBC 884 and UBC 850 primers showed optimal band numbers and good band intensities. Sixty isolates were evaluated with these 8 ISSR primers. UBC826 and UBC886 primers amplified the largest numbers of bands. The genetic diversity of the isolates in the R. commune population in Turkey appeared to be low. There were no definite groupings of the mating type or geographical region. Turkey is one of the gene centers of barley and this study is the first one addressing the genetic variation and mating type distribution of R. commune in Turkey. The low genetic diversity and possible asexual reproduction observed in our study may contribute to the hypothesis that R. commune did not evolve in the origin of the barley plant.



中文翻译:

土耳其Rhynchosporium comumune的遗传变异和交配类型分布

鼻孢子虫公社是大麦烫伤病的病原体,是世界范围内重要的大麦叶面病。在2012年,2013年和2014年期间,从土耳其不同地区收集了Rhynchosporium commune单孢子分离株,并从中选择了60个分离株。确定了这些选定菌株的交配类型分布和流行程度。二十一个的60个单孢子分离物被发现是MAT1-1型和39个分离物MAT1-2型χ 2(1:1)类型= 5.40(P = 0.02)。交配类型比率表明,R。com.une种群在土耳其可能主要是无性繁殖。确定公社的遗传多样性分离物,来自土耳其不同地区的5个单孢子分离物用72种ISSR引物进行了测试。发现11个引物是多态的。UBC 826,UBC 861,UBC 886,UBC 887,UBC 890,UBC 848,UBC 884和UBC 850引物显示出最佳条带数量和良好的条带强度。用这8个ISSR引物评估了60个分离株。UBC826和UBC886引物扩增出最多的条带。土耳其R. com.une种群中分离株的遗传多样性似乎较低。没有交配类型或地理区域的明确分组。土耳其是大麦的基因中心之一,这项研究是第一个针对大麦公羊的遗传变异和交配类型分布的研究在土耳其。在我们的研究中观察到的低遗传多样性和可能的​​无性繁殖可能有助于假设大麦植物起源时公社不进化。

更新日期:2021-02-08
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