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Cyclic voltammetric biosensing of cellular ionic secretion based on silicon nanowires to detect the effect of paclitaxel on breast normal and cancer cells
Microelectronic Engineering ( IF 2.3 ) Pub Date : 2021-01-29 , DOI: 10.1016/j.mee.2021.111512
Hani Shashaani , Navid Akbari , Mahsa Faramarzpour , Mohammad Salemizadeh Parizi , Shohreh Vanaei , Mohammad Ali Khayamian , Mohammad Faranoush , Robab Anbiaee , Mohammad Abdolahad

Cell-free analysis based on environmental secretions of the cells/tissues, such as point of care testing, is a rapidly evolving area with advances in integrating sample handling, cheap fabrication, and easy operation. Also, they could be used as complementary cell-based analysis without inducing any perturbation on standard assays. Here, we present a secretion-based cyclic voltammetry biosensor with the assistance of silicon nanowire electrodes to detect ionic concentration changes in the secretions of breast normal and cancer cell lines after being treated by Paclitaxel drug. Changes in CV peaks of the secretions showed a meaningful correlation with the levels of apoptosis after drug treatment in both healthy and malignant cells. Such changes were sharper in MDA-MB-231 than MCF-10A cell lines. This platform could be applied as a cell-free apoptosis sensor to monitor the drug resistance of cancer cells. The sensor is not single used and could be re-used by a simple rinse protocol.



中文翻译:

基于硅纳米线的细胞离子分泌的循环伏安法生物传感,以检测紫杉醇对乳腺癌正常细胞和癌细胞的作用

基于细胞/组织的环境分泌物的无细胞分析(例如即时检验)是一个快速发展的领域,在整合样品处理,廉价制造和易于操作方面取得了进步。而且,它们可以用作基于细胞的补充分析,而不会在标准测定中引起任何干扰。在这里,我们提出了一种基于分泌的循环伏安法生物传感器,借助硅纳米线电极来检测紫杉醇药物治疗后正常乳腺和癌细胞系分泌物中的离子浓度变化。在健康和恶性细胞中,分泌物的CV峰的变化与药物治疗后的细胞凋亡水平均显示出有意义的相关性。与MCF-10A细胞系相比,MDA-MB-231中的这种变化更为明显。该平台可用作无细胞凋亡传感器,以监测癌细胞的耐药性。该传感器不是一次性使用的,可以通过简单的冲洗规程重复使用。

更新日期:2021-03-03
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