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Physiological responses to live air transport of red king crab (Paralithodes camtschaticus)
Fisheries Research ( IF 2.4 ) Pub Date : 2021-01-29 , DOI: 10.1016/j.fishres.2021.105882
Vasco C. Mota , Sten I. Siikavuopio , Philip James

Live transport of red king crab from the fishing grounds to the market is an activity that is increasing and the future sustainability of this practice depends on maintaining optimal animal welfare during transport. The current study evaluated the physiological response of red king crab (Paralithodes camtschaticus) to live air transport, mimicking current commercial practices. Specifically, this study assessed stress and osmoregulatory haemolymph metabolite variation, together with free amino acids (FAAs) pool variation in the haemolymph of red king crab. Forty male crabs (2136 ± 800 g) were caught in the North Cape area in the Barents Sea during April 2019. They were transported by the fishing boat directly to a land-based live holding facility in Honningsvårg, Norway where they were held for < 10 days. They were then divided in two groups: i) the first group of 20 crabs underwent immediate haemolymph sampling, and ii) the second group of 20 crabs was divided in subgroups of 5 crabs, placed in a standard air freight Styrofoam boxes with freezer packs and transported by road and air to the Nofima facility, in Tromsø, Norway. The time from the packing until when the haemolymph sampling occurred was 20 h. All samples of haemolymph were taken from the coxa membrane of the third right side walking leg and were analysed for sodium (Na+), chloride (Cl), carbon dioxide (CO2), lactate, total protein, and FAAs. The survival of the transported crabs was 100 %, and after transport the measured indicators showed a significantly positive (lactate and total protein) or a negative (Na+, Cl) variation in relation to the air transport. From the measured FAAs, five, Alanine (Ala), Arginine (Arg), Threonine (Thr), Valine (Val) and Taurine (Tau), were significantly higher for the group after transport when compared to the group before transport. No FAAs were significantly lower as a result of transport. In conclusion, it was apparent that red king crabs exposed to air during a 20 -h transport period exhibited the typical physiological responses associated to hypoxia stress. Moreover, in this study we report for the first time a change in the FAA pool results due to transport where five out of the twenty-one measured FAA where significantly elevated. Specifically, Ala, an important osmolyte during salinity stress. These results bring new insights into how red king crabs cope with live air transport and offer new evidence for the need to assess the crabs’ health and welfare during live holding and transport. Moreover, the results obtained from this study can also contribute to the development of an industry friendly methodology to assess the stress and health status of crabs during and after live transport.



中文翻译:

红帝王蟹(Paralithodes camtschaticus)对空气传播的生理响应。

从捕鱼场到市场的红色帝王蟹的现场运输是一项正在增加的活动,这种作法的未来可持续性取决于在运输过程中保持最佳的动物福利。当前的研究评估了红帝王蟹(Paralithodes camtschaticus)的生理反应)进行实时航空运输,模仿当前的商业惯例。具体来说,本研究评估了红帝王蟹的血淋巴中的压力和渗透调节性血淋巴代谢物变异以及游离氨基酸(FAA)库变化。在2019年4月,四十只雄性蟹(2136±800克)在巴伦支海的北开普地区被捕。它们被渔船直接运到挪威洪宁斯沃格的一个陆上生物捕捞设施,在那里被捕< 10天。然后将它们分为两组:i)第一组的20只螃蟹立即进行了血淋巴取样; ii)第二组的20只螃蟹分为5个螃蟹的亚组,放在带有冷冻包装的标准空运聚苯乙烯泡沫塑料盒中,以及通过陆路和空中运输到位于挪威Tromsø的Nofima工厂。从包装到发生血淋巴取样的时间为20小时。血淋巴的所有样本均取自右第三只步行小腿的结肠膜,并进行了钠(Na+),氯(氯- ),二氧化碳(CO 2),乳酸,总蛋白,和FAAS。被输送的螃蟹的存活率为100%,和运输后所测量的指标呈显著正(乳酸和总蛋白)或否定(钠+,氯-)与航空运输有关的变化。从测量的FAA中,与运输前的组相比,运输后的组中的五个分别为:丙氨酸(Ala),精氨酸(Arg),苏氨酸(Thr),缬氨酸(Val)和牛磺酸(Tau)。由于运输,没有FAA明显降低。总之,很明显,在运输20小时的过程中暴露在空气中的红帝王蟹表现出与缺氧应激相关的典型生理反应。此外,在本研究中,我们首次报告了由于运输而导致的FAA池结果的变化,其中21个测量FAA中有5个显着升高。具体来说,丙氨酸是盐度胁迫期间的重要渗透压。这些结果为红帝王蟹如何应对空中运输提供了新的见解,并为评估在活体运输过程中螃蟹的健康和福利提供了新的证据。此外,从这项研究中获得的结果还可以有助于开发一种行业友好的方法,以评估活体运输过程中和运输后螃蟹的压力和健康状况。

更新日期:2021-01-29
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