Crop Protection ( IF 2.8 ) Pub Date : 2021-01-29 , DOI: 10.1016/j.cropro.2021.105559 Abd Rahim Huda-Shakirah , Masratul Hawa Mohd
The occurrence of leaf blotch of orchid (Dendrobium antennatum) was observed in two locations in Sungai Petani, Kedah, Malaysia and Sungai Dua, Penang, Malaysia. Two isolates of Fusarium sp. were isolated from the infected leaves. Based on morphological characteristics and comparison of DNA sequences of translation elongation factor 1-alpha (TEF1-α) and mitochondrial small subunit ribosomal DNA (mtSSU rDNA), the isolated fungal isolates were identified as Fusarium sacchari. Phylogenetic analysis of the combined dataset of TEF1-α and mtSSU showed that the isolates were grouped with the reference isolates of F. sacchari from GenBank database. The pathogenicity test revealed both isolates of F. sacchari responsible for causing leaf blotch of D. antennatum. This is the first report of F. sacchari causing leaf blotch of D. antennatum in Malaysia and worldwide.
中文翻译:
糖镰刀菌在马来西亚引起兰花(石end触角)叶片斑点的首次报道
在马来西亚吉打州双溪大年和马来西亚槟城双溪杜阿的两个地方都观察到了兰花(石end触角)叶斑的发生。镰刀菌属的两个分离株。从受感染的叶子中分离出来。根据形态特征并比较翻译延伸因子1-alpha(TEF1-α)和线粒体小亚基核糖体DNA(mtSSU rDNA)的DNA序列,鉴定出分离的真菌分离株为糖镰刀菌。TEF1-α和mtSSU的组合数据集的系统发育分析表明,分离物用的参考菌株分组F.甘蔗从GenBank数据库。致病性测试显示两个分离的糖酵母造成触角藻叶片斑点的原因。这是首次报告F.甘蔗引起的叶斑病D. antennatum在马来西亚和世界各地。