Abstract

Polyvinylpyrrolidone (PVP) is a biopolymer compound that has been widely used in pharmaceutical and medical applications, especially as a tablet binder and solubilizing agents for injections. In this work, PVP was characterized by FTIR to confirm its structure and Dynamic light scattering (DLS) to determine its mean diameter for studying concentration ranges. PVP was radio-labeled with Technetium-99m using SnCl₂.2H₂O as a reducing agent with an efficiency of (97 ± 0.15) % at the best conditions. The stability ^99mTc-PVP complex was measured in serum and saline media in vitro for 24 hours and it was found that the complex had good stability properties for 24 hours. The biological distribution of the ^99mTc-PVP complex was studied in normal mice using oral and intravenous injection to discover its accumulation in different mice organs. The results indicated that the rate of the ^99mTc-PVP complex in the blood decreased rapidly during the first three hours after injection, and also, it was mainly excreted by the kidneys with negligible uptake in the brain. The results also confirmed that the ^99mTc-PVP complex was accumulated with high percentages in the urinary bladder during the 3 hours post-injection.

摘要

聚乙烯吡咯烷酮（PVP）是一种生物聚合物化合物，已广泛用于制药和医疗应用，尤其是用作片剂粘合剂和注射用增溶剂。在这项工作中，通过FTIR对PVP进行表征，以确认其结构，并通过动态光散射（DLS）确定其平均直径以研究浓度范围。使用Technetium-99m使用SnCl ₂ .2H ₂ O作为还原剂对PVP进行放射性标记，在最佳条件下的效率为（97±0.15）％。在体外血清和盐溶液中测定24小时的稳定性^99m Tc-PVP复合物，发现该复合物在24小时内具有良好的稳定性。^99m的生物分布在口服和静脉注射的正常小鼠中研究了Tc-PVP复合物，以发现其在不同小鼠器官中的积累。结果表明，在注射后的前三个小时内，血液中^99m Tc-PVP复合物的比率迅速下降，并且主要由肾脏排泄，而在大脑中的吸收可忽略不计。结果还证实，在注射后3小时内，膀胱中的^99m Tc-PVP复合物以高百分比积累。