The detection and quantification of nucleic acid and proteomic biomarkers in bodily fluids is a critical part of many medical screening and diagnoses. However, majority of the current detection platforms are not ideal for routine, rapid, and low‐cost testing in point‐of‐care settings. To address this issue, we developed a concept for a disposable universal point‐of‐care biosensor that can detect and quantify nucleic acid and proteomic biomarkers in diluted serum samples. The central tenet of sensing is the use of dielectrophoresis, electrothermal effects, and thermophoresis to selectively and rapidly isolate the biomarkers of interest in electrodes and then quantify using electrical impedance. When the sensor was applied to quantify microRNA and antigen biomarker molecules directly in diluted serum samples, it produced a LOD values in the fM range and sensitivity values from 10¹² to 10¹⁵ Ω/M with a 30 min assay time and assay cost of less than $50 per assay.

中文翻译：

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集成介电泳和阻抗生物传感器为临床样本中的通用生物标志物传感提供了模板

体液中核酸和蛋白质组生物标志物的检测和定量是许多医学筛查和诊断的关键部分。然而，当前的大多数检测平台对于床旁环境中的常规、快速和低成本检测并不理想。为了解决这个问题，我们开发了一种一次性通用即时生物传感器的概念，该传感器可以检测和量化稀释血清样本中的核酸和蛋白质组生物标志物。传感的中心原则是使用介电泳、电热效应和热泳来选择性地和快速地分离电极中感兴趣的生物标志物，然后使用电阻抗进行量化。当传感器用于直接量化稀释血清样本中的 microRNA 和抗原生物标志物分子时，¹²至 10 ¹⁵ Ω/M，测定时间为 30 分钟，每次测定的测定成本低于 50 美元。