The morphological changes of phosphonate polypeptoid electrolyte membranes, poly‐N‐(2‐ethyl)hexylglycine‐block‐poly‐N‐phosphonomethylglycine (pNeh_m‐b‐pNpm_n), in hydrated and dry states were characterized by cryogenic transmission electron microscopy (cryo‐TEM) and cryogenic electron tomography (cryo‐ET). The analysis of 3D tomograms revealed that the pNeh₉‐b‐pNpm₉ thin films absorbed a large amount of water, resulting in the formation of membranes that were nearly flat and giant multicompartment vesicles dispersed in the water phase. A simple lamellar phase appeared when the films were dried. In contrast, pNeh₁₈‐b‐pNpm₁₈ thin films absorbed little water and formed small highly curved unilamellar and multilamellar vesicles. Water was located mainly outside the closely‐packed vesicles. When water was removed by drying, the walls of adjacent vesicles collapsed to form honeycomb‐like capsules. The changes in domain size reflected changes in chain conformations. The pNpm₉ blocks were saturated by water and fully extended, while pNpm₁₈ blocks were neither saturated by water nor fully extended. In addition, the thicknesses of hydrophobic blocks in the hydrated films of both pNeh₉‐b‐pNpm₉ and pNeh₁₈‐b‐pNpm₁₈ were smaller than those in the dry films, reflecting an increase of the average distance between the neighboring junctions of polypeptoid molecules.

中文翻译：

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电子断层研究水合对膦酸酯嵌段共聚物电解质薄膜的形貌的影响

的形态学改变膦酸盐polypeptoid电解质膜，聚- ñ - （2-乙基）hexylglycine-块-聚Ñ -phosphonomethylglycine（pNeh_米- b -pNpm _Ñ）通过低温透射电子显微镜，在水合和干燥状态进行了表征（低温TEM）和低温电子断层扫描（cryo-ET）。对3D断层图的分析显示pNeh ₉ ‐ b ‐ pNpm ₉薄膜吸收了大量的水，导致形成几乎平坦的膜，并在水相中散布着巨大的多室囊泡。当膜干燥时，出现简单的层状相。相比之下，pNeh ₁₈ - b- pNpm ₁₈薄膜吸收的水分很少，并形成小的高度弯曲的单层和多层囊泡。水主要位于密堆积的囊泡外部。当通过干燥除去水时，相邻囊泡的壁塌陷形成蜂窝状胶囊。域大小的变化反映了链构象的变化。pNpm ₉块被水饱和并充分延伸，而pNpm ₁₈砌块既没有被水浸透也没有完全伸展。此外，pNeh ₉ ‐ b ‐ pNpm ₉和pNeh ₁₈ ‐ b ‐ pNpm ₁₈的水合膜中疏水性嵌段的厚度均小于干膜中的疏水性嵌段的厚度，这反映了相邻膜之间平均距离的增加。多肽分子。