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Synthesis of Bioethanol from Oat Husk via Enzyme-Substrate Feeding
Catalysis in Industry Pub Date : 2021-01-27 , DOI: 10.1134/s2070050420040054
G. F. Mironova , E. A. Skiba

Abstract

A promising way of utilizing oat husks is its bioconversion into technical bioethanol. Ways of increasing the concentration of bioethanol in the mash must be found to increase the economic efficiency of the process. A combination of enzymatic hydrolysis and alcoholic fermentation (combined biocatalytic stages, or CBS) can be used to obtain bioethanol. In addition, delayed introduction of the inoculum can be used when combining stages. In this work, a comparative study is performed of the CBS processes with an initial substrate concentration of 60 g/L (one-time substrate loading) and an increased substrate concentration of 120 g/L after enzyme–substrate feeding (loading the substrate at a concentration of 60 g/L at the beginning of the process and feeding 30 g/L of the substrate after 4 and 8 hours. The substrate is oat husks treated with 4 wt % nitric acid in a pilot plant; a multi-enzyme composition of industrial preparations Cellolux-A and Ultraflo Core is used for enzymatic hydrolysis. Alcohol is fermented using the yeast Saccharomyces сerevisiae Y-1693 (VKPM). The equipment is a fermenter with a capacity of 11 L. Feeding with a doubled concentration of the substrate raises the concentration of bioethanol by 1.7 times (from 2.4 to 4.0 vol %) but lowers its yield by 11.4%. Make-up regimes are being developed that ensure higher concentrations of bioethanol in the mash without reducing its yield.



中文翻译:

燕麦底壳通过酶-底物进料合成生物乙醇

摘要

利用燕麦壳的一种有前途的方法是将其生物转化为工业用生物乙醇。必须找到增加the液中生物乙醇浓度的方法,以提高该方法的经济效率。酶促水解和酒精发酵的组合(组合的生物催化阶段,或CBS)可用于获得生物乙醇。另外,组合阶段时可以使用接种物的延迟引入。在这项工作中,对CBS工艺进行了比较研究,初始底物浓度为60 g / L(一次性底物加载),酶-底物进料后(底物加载为在工艺开始时的浓度为60 g / L,并在4和8小时后进料30 g / L的底物。基质是中试工厂中用4 wt%硝酸处理过的燕麦壳;工业制剂Cellolux-A和Ultraflo Core的多酶组合物用于酶促水解。用酵母发酵酒精酿酒酵母 Y-1693(VKPM)。该设备是一台容量为11 L的发酵罐。进料量加倍的底物可使生物乙醇的浓度提高1.7倍(从2.4到4.0体积%),但产率降低11.4%。正在开发可确保糖化mash中更高浓度的生物乙醇而不降低其收率的补充方案。

更新日期:2021-01-28
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