Butyrylcholinesterase (BChE) is an essential human cholinesterase relevant to liver conditions and neurodegenerative diseases, which makes it a pivotal biomarker of health. It therefore remains challenging and highly desired to elaborate efficient chemical tools for BChE with simple operations and satisfactory working performance. In this work, a background-free detection strategy was built by virtue of the judicious coupling of a specific BChE-enzymatic reaction and in situ cyclization. High sensitivity with a low limit of detection (LOD) of 0.075 μg/mL could be readily achieved from the blank background and the as-produced emissive indicators, and the specific reaction site contributed to the high selectivity over other bio-species even acetylcholinesterase (AChE). In addition to the multifaceted spectral experiments to verify the sensing mechanism, this work assumed comprehensive studies on the application. The bio-investigation ranged from cells to an organism, declaring a noteworthy prospect in disease diagnosis, especially for Alzheimer’s disease (AD), a common neurodegenerative disease with over-expressed BChE. Moreover, its excellent work for inhibition efficacy elucidation was also proved with the accuracy IC₅₀ of tacrine for BChE (8.6 nM), giving rise to an expanded application for trace pesticide determination.

中文翻译：

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荧光测定丁酰胆碱酯酶活性及其在生物成像和农药残留检测中的应用

丁酰胆碱酯酶（BChE）是与肝脏疾病和神经退行性疾病相关的必不可少的人胆碱酯酶，这使其成为健康的关键生物标志物。因此，仍然具有挑战性，并且迫切需要开发出操作简单且令人满意的工作性能的高效BChE化学工具。在这项工作中，借助特定BChE酶促反应与原位环化的明智结合，建立了无背景检测策略。从空白背景和产生的发光指示剂可以轻松实现0.075μg/ mL的低检测限（LOD）的高灵敏度，并且特定的反应位点对其他生物物种（甚至是乙酰胆碱酯酶（疼痛）。除了用于验证传感机制的多方面光谱实验之外，这项工作还对应用进行了全面研究。生物研究的范围从细胞到生物，在疾病诊断方面都具有显着的前景，特别是对于阿尔茨海默氏病（AD），这是一种过度表达BChE的常见神经退行性疾病。此外，精度IC还证明了其在抑制功效阐明方面的出色工作。_50克他克林用于BChE（8.6 nM），扩大了痕量农药测定的应用范围。