A biosurfactant producing strain was isolated and the rhamnolipid type biosurfactant was extracted for soil washing of a synthetically and naturally hydrocarbon-contaminated soil. Following the primary screening, Pseudomonas aeruginosa strain R₄ was selected and the effect of the carbon and nitrogen source and the salinity on biosurfactant production was studied. Of the best results were observed for glucose as a carbon source, NH₄Cl as a nitrogen source and salinity of 1.4%. The produced biosurfactant was a glycolipid type biosurfactant and reduced the surface tension to 32.5 mN/m with a critical micelle concentration (CMC) of 50 mg/L and production yield of 90 mg/L. Using produced biosurfactant, a pyrene desorption rate of 82% was observed in selected conditions for initial pyrene concentration of 200 mg/L.

分离出一株产生生物表面活性剂的菌株，并提取鼠李糖脂类生物表面活性剂，用于对合成和天然碳氢化合物污染土壤进行土壤洗涤。初步筛选后，筛选出铜绿假单胞菌菌株R ₄，研究了碳源、氮源和盐度对生物表面活性剂生产的影响。以葡萄糖为碳源的最佳结果中，NH ₄以Cl为氮源，盐度为1.4%。制备的生物表面活性剂为糖脂型生物表面活性剂，表面张力降低至32.5 mN/m，临界胶束浓度（CMC）为50 mg/L，产率为90 mg/L。使用生产的生物表面活性剂，在初始芘浓度为 200 mg/L 的选定条件下观察到芘解吸率为 82%。