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Royal jelly may improve sperm characteristics during preservation of rooster semen: Gene expression of antioxidant enzymes
Reproduction in Domestic Animals ( IF 1.7 ) Pub Date : 2021-01-27 , DOI: 10.1111/rda.13902
Atefeh Hadavand Mirzaei 1 , Hamid Deldar 1 , Zarbakht Ansari Pirsaraei 1 , Bahram Shohreh 1
Affiliation  

Sustainable production and the increasing number of embryonated hatching eggs are critical aspects of the poultry production industry. The present paper aims to appraise the effectiveness of royal jelly (RJ) on the semen characteristics of Native Mazandaran roosters in both liquid and frozen storage conditions. Semen collected from 10 sexually mature roosters and following dilution was supplemented with RJ at 0.0 (control), 5 (RJ5), 10 (RJ10), 20 (RJ20) and 40 (RJ 40) mg/ml. After cooling and freezing‐thawing, the percentage of forward progressive motility, viability, abnormality, hypo‐osmotic swelling test (HOST) and the mRNA abundance of antioxidant enzymes of spermatozoa were measured. Our results revealed that the addition of 5 mg/ml RJ to the semen extender significantly increased (p < .05) the percentages of forward progressive motility, viability and HOST during liquid and frozen storage. The abnormality of spermatozoa in the RJ5 group was significantly lower compared to the other groups. During liquid storage, a significant decrease in forward progressive motility was found after 48 hr in comparison with 24 hr at 4°C. High levels of RJ (from 10 to 40 mg/ml) were severely decreased the characteristics of rooster spermatozoa in comparison with RJ5 and the control group. The inclusion of RJ at 5 mg/ml to the semen extender enhanced the mRNA transcript of antioxidant enzymes of spermatozoa during liquid preservation. The mRNA abundance of antioxidant enzymes did not influence by cryostorage. Overall, these data suggest that supplementation of RJ at 5 mg/ml to the extender improved semen characteristics and redox status of rooster spermatozoa.

中文翻译:

蜂王浆可在保存公鸡精液过程中改善精子特性:抗氧化酶的基因表达

可持续生产和越来越多的含胚孵化蛋是家禽生产行业的关键方面。本论文旨在评估蜂王浆 (RJ) 在液体和冷冻储存条件下对原生马赞德兰公鸡精液特征的有效性。从 10 只性成熟公鸡收集并稀释后的精液中添加了 0.0(对照)、5(RJ5)、10(RJ10)、20(RJ20)和 40(RJ 40)mg/ml 的 RJ。冷却和冻融后,测量精子向前进行性运动、活力、异常、低渗肿胀试验(HOST)和抗氧化酶mRNA丰度的百分比。我们的结果显示,向精液补充剂中添加 5 mg/ml RJ 显着增加(p < .05) 液体和冷冻储存过程中前向运动性、生存力和宿主的百分比。RJ5组精子异常明显低于其他组。在液体储存期间,与 4°C 下的 24 小时相比,48 小时后前向运动性显着降低。与 RJ5 和对照组相比,高水平的 RJ(从 10 到 40 毫克/毫升)严重降低了公鸡精子的特征。将 5 毫克/毫升的 RJ 加入到精液稀释剂中可增强液体保存过程中精子抗氧化酶的 mRNA 转录。抗氧化酶的 mRNA 丰度不受冷冻保存的影响。总体,
更新日期:2021-01-27
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