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Widely-tunable synchronisation-free picosecond laser source for multimodal CARS, SHG, and two-photon microscopy
Biomedical Optics Express ( IF 3.4 ) Pub Date : 2021-01-26 , DOI: 10.1364/boe.411620
Duanyang Xu , Sijing Liang , Lin Xu , Konstantinos N. Bourdakos , Peter Johnson , James Read , Jonathan H. V. Price , Sumeet Mahajan , David J. Richardson

We demonstrate a continuous wave (CW) seeded synchronization-free optical parametric amplifier (OPA) pumped by a picosecond, 1 µm laser and show its performance when used as a simple yet powerful source for label-free coherent anti-Stokes Raman scattering (CARS), concurrent second harmonic generation (SHG), and two-photon fluorescence microscopy in an epi-detection geometry. The average power level of above 175 mW, spectral resolution of 8 cm−1, and 2 ps pulse duration are well optimized for CARS microscopy in bio-science and bio-medical imaging systems. Our OPA is a much simpler setup than either the “gold-standard” laser and optical parametric oscillator (OPO) combination traditionally used for CARS imaging, or the more recently developed OPA systems pumped with femtosecond pulses [1]. Rapid and accurate tuning between resonances was achieved by changing the poled channels and temperature of the periodically-poled lithium niobate (PPLN) OPA crystal together with the OPA seed wavelength. The Pump-Stokes frequency detuning range fully covered the C-H stretching band used for the imaging of lipids. By enabling three multiphoton techniques using a compact, synchronization free laser source, our work paves the way for the translation of label-free multi-photon microscopy imaging from biomedical research to an imaging based diagnostic tool for use in the healthcare arena.

中文翻译:

广泛可调的无同步皮秒激光源,用于多模式CARS,SHG和双光子显微镜

我们演示了由皮秒,1 µm激光器泵浦的连续波(CW)种子无同步光参量放大器(OPA),并展示了其在用作无标签相干抗斯托克斯拉曼散射(CARS)的简单而强大的光源时的性能),并发二次谐波产生(SHG)和Epi检测几何中的双光子荧光显微镜。平均功率高于175 mW,光谱分辨率为8 cm -1对于生物科学和生物医学成像系统中的CARS显微镜,2 ps脉冲持续时间已得到最佳优化。与传统上用于CARS成像的“金标准”激光和光学参量振荡器(OPO)组合,或者较新开发的带有飞秒脉冲的OPA系统相比,我们的OPA设置要简单得多。通过改变周期性极化的铌酸锂(PPLN)OPA晶体的极化通道和温度以及OPA种子波长,可以实现谐振之间的快速,准确的调谐。Pump-Stokes频率失谐范围完全覆盖了用于脂质成像的CH拉伸带。通过使用紧凑的无同步激光源启用三种多光子技术,
更新日期:2021-02-01
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