In this work, a time-gated immunoassay platform using low-energy excitable and fluorescence long-lived Mn:AgZnInS/ZnS nanocrystals as signal transducers was developed and applied to the detection of the capsular polysaccharide (CPS) of Burkholderia pseudomallei, a Gram-negative bacterium that is the causative agent of melioidosis. CPS is a high molecular weight antigen displayed and is shed from the outer membrane of B. pseudomallei. The immunoassay using the time-gated platform presents a limit of detection at around 23 pg/ml when CPS is spiked in human serum.

中文翻译：

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锰掺杂的AgZnInS / ZnS纳米晶体作为信号传感器的高灵敏时间门控荧光免疫测定平台。

在这项工作中，开发了一种使用低能量可激发和荧光长寿命Mn：AgZnInS / ZnS纳米晶体作为信号传感器的门控免疫测定平台，并将其用于检测肝细胞的荚膜多糖（CPS） 假伯克霍尔德氏菌，是革兰氏阴性菌的革兰氏阴性细菌。CPS是一种展示的高分子量抗原，从CPS的外膜脱落假单胞菌。当在人血清中加标CPS时，使用时间门控平台的免疫测定法的检测极限约为23 pg / ml。