ABSTRACT

The aim of this study was to phenotypically and genotypically identify coagulase-negative Staphylococcus (CoNS) recovered from the nostrils of waste workers and from dental waste; 135 strains were recovered and S. epidermidis was the prevailing species. Genetic similarity (100%) was observed between the two S. epidermidis isolated from different employees on the same shift and 85% similarity between the S. epidermidis recovered from an employee’s nostril and from waste. The mecA gene was found in 20 CoNS, and 20% were also found to possess the vanA gene. The blaZ gene was detected in 46.7%, and the icaA (34.8%), B and C genes (11.8% each). Our findings emphasized the biological risk to which waste workers are exposed and unprecedently confirms that it was possible to recover genetically identical bacterial species from waste and from workers’ nostrils. It is important to highlight that this risk is raised by the detection of relevant antimicrobial resistance genes. The results also suggest that effective measures to correctly manage waste and promote the rational use of antimicrobials should be adopted.

摘要

本研究的目的是从表型和基因型上鉴定从废物工人的鼻孔和牙科废物中回收的凝固酶阴性葡萄球菌(CoNS)；回收菌株135株，以表皮葡萄球菌为主。从同一班次的不同员工分离的两个表皮葡萄球菌之间观察到遗传相似性 (100%)，从员工的鼻孔和废物中回收的表皮葡萄球菌之间的相似性为 85%。在20 个 CoNS 中发现了mecA基因，并且还发现 20% 具有vanA基因。blaZ基因在 46.7% 中被检测到，而icaA（34.8%）、B和C基因（各11.8%）。我们的研究结果强调了废物工人所面临的生物风险，并且史无前例地证实了从废物和工人的鼻孔中恢复基因相同的细菌物种是可能的。需要强调的是，检测相关抗菌素耐药基因会增加这种风险。结果还表明，应采取有效措施正确管理废物，促进抗菌药物的合理使用。