Andrographis alata (Vahl) Nees is a medicinal plant which was reported to have the highest concentration of neoandrographolide that has several therapeutic values. Natural populations of Andrographis alata are dwindling due to destruction of natural habitat and over exploitation. Therefore, in vitro propagation of Andrographis alata was undertaken, and successful method is presented here. Micropropagation of Andrographis alata was realized on MS nutrient medium augmented with BAP (10 μM), and multiple shoots were regenerated from nodal explants. Induction of roots was attained from shoots on ¼ concentration of MS nutrient medium supplemented with IBA (1 μM). Randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) analysis showed that there is genetic fidelity in the regenerated plants. Reverse phase high performance liquid chromatographic analysis of regenerated plants showed the presence of neoandrographolide, equivalent to that of mother plants. Successful in vitro regeneration of Andrographis alata is presented here, and it is quite useful for its mass multiplication. The micropropagated plants are useful for restoration of plants in nature and for utilization by the pharmaceutical industry for extraction of neoandrographolide.

中文翻译：

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快速植物再生，遗传保真度的分析，以及微量繁殖的植物的新穿心莲内酯含量穿心莲翅（干旱胁迫下连翘）孢

穿心莲（Vahl）Nees是一种药用植物，据报道具有最高浓度的新穿心莲内酯具有多种治疗价值。由于对自然栖息地的破坏和过度开发，穿心莲的自然种群正在减少。因此，进行了穿心莲的体外繁殖，并在此提出了成功的方法。在添加BAP（10μM）的MS营养培养基上实现穿心莲的微繁殖，并从结节外植体中再生出许多芽。芽的根诱导是在1/4浓度的补充有IBA（1μM）的MS营养培养基上进行的。随机扩增的多态性DNA（RAPD）和简单序列间重复（ISSR）分析表明，再生植物具有遗传保真度。再生植物的反相高效液相色谱分析显示新穿心莲内酯的存在与母体植物相当。这里展示了穿心莲的成功体外再生，并且对于其大量繁殖非常有用。微繁殖的植物可用于自然界中的植物的恢复，以及可用于制药工业中提取新穿心莲内酯。