Gel image analysis is a cornerstone in electrophoresis-based experiments. Nature of the experiment determines the target of the analysis. Implemented features within different software for gel images and their outputs are variable. EgyGene GelAnalyzer4 software aimed to make a powerful, easy, and almost all-in-one software for gel image analysis. EgyGene GelAnalyzer4 is a software for analyzing the gel images. The program consists of six main parts: gel image enhancement, image analysis, data merging, molecular markers detection, phylogenetic tree prediction, and population parameters estimation. Firstly, the gel image enhancement part enhances the gel image colors and clarity. Then, the image is analyzed in the second part using both automatic and manual detection of lanes and bands. The third part, data merging, is for merging several analyses for different samples/gels together to produce one table comprises the entire experiment. The fourth section, markers detection, collects several pre-analyzed data for the same samples to detect their molecular markers based on some user-defined criteria. The fifth part uses the outputs of the previous parts to draw the phylogenetic tree between samples. The program constructs a phylogenetic tree using unweighted pair group method with arithmetic mean (UPGMA) method. Fourteen different equations are used to calculate the similarity percentages: Anderberg, Czekanowski, Dice, Faith, Gower and Legendre, Jaccard, Nei and Li, Rogers and Tanimoto, Russel and Rao, simple matching, Sokal and Michener, Sokal and Sneath 1, Sokal and Sneath 2, and Sorensen. The last section calculates the population parameters for the pre-analyzed images before. EgyGene GelAnalyzer4 is designed and programed using Microsoft Visual Studio 2012 Express version, based on the Dot Net FrameWork 4.5 edition, whereas its icons are created using the freeware Greenfish Icon Editor Pro 3.2.5.1. EgyGene GelAnalyzer4 is a simple, fast, and user-friendly software with six novel features: changing the order of lanes within the gel image, splitting the detecting lane into two lanes, merging of several detected lanes into just one, splitting a single band into two bands, merging two or more bands to be a single band, and comparing data of several analyzed images with a big number of samples and merging it together in a single table and detection of molecular markers between samples. In addition to the basic features and outputs of other image analysis software such as calculation of relative mobility values, molecular weights/sizes estimation, DNA/RNA/protein quantity within bands, table of present/absent of bands, polymorphism percentage, similarity percentages and phylogeny, population parameters, enhancing the image colors and clarity, and merging data for several gels. In addition, writing names of samples and the detected markers on the image. EgyGene GelAnalyzer4 provides many several features, which may be not available together in any other alternative.

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EgyGene GelAnalyzer4：用于一维凝胶电泳的功能强大的图像分析软件

凝胶图像分析是基于电泳的实验的基石。实验的性质决定了分析的目标。不同软件中用于凝胶图像及其输出的已实现功能是可变的。EgyGene GelAnalyzer4软件旨在制作功能强大，简单易用的几乎所有功能的凝胶图像分析软件。EgyGene GelAnalyzer4是用于分析凝胶图像的软件。该程序包括六个主要部分：凝胶图像增强，图像分析，数据合并，分子标记检测，系统树预测和种群参数估计。首先，凝胶图像增强部分增强了凝胶图像的颜色和清晰度。然后，在第二部分中使用自动和手动检测车道和车道来分析图像。第三部分，数据合并 用于将不同样品/凝胶的几种分析合并在一起以产生一张包含整个实验的表格。第四部分，标记检测，收集一些相同样品的预分析数据，以根据一些用户定义的标准检测其分子标记。第五部分使用前面各部分的输出来绘制样本之间的系统发育树。该程序使用具有算术平均值的非加权对组方法（UPGMA）构造系统发育树。使用十四个不同的方程式计算相似度百分比：Anderberg，Czekanowski，Dice，Faith，Gower和Legendre，Jaccard，Nei和Li，Rogers和Tanimoto，Russel和Rao，简单匹配，Sokal和Michener，Sokal和Sneath 1，Sokal还有Sneath 2和Sorensen。最后一部分计算之前的预分析图像的总体参数。EgyGene GelAnalyzer4是使用Microsoft Visual Studio 2012 Express版本（基于Dot Net FrameWork 4.5版本）设计和编程的，而其图标是使用免费软件Greenfish Icon Editor Pro 3.2.5.1创建的。EgyGene GelAnalyzer4是一款简单，快速且用户友好的软件，具有六个新颖功能：更改凝胶图像中泳道的顺序，将检测泳道分为两个泳道，将多个检测泳道合并为一个泳道，将单个谱带分为两个波段，将两个或多个波段合并为一个波段，然后将多个分析图像的数据与大量样本进行比较，然后将其合并到一张表格中，并检测样本之间的分子标记。除了其他图像分析软件的基本功能和输出外，例如相对迁移率值的计算，分子量/大小估计，条带内的DNA / RNA /蛋白质数量，条带的存在/缺失表，多态性百分比，相似性百分比和系统发育，种群参数，增强图像的颜色和清晰度以及合并几种凝胶的数据。另外，在图像上写上样品名称和检测到的标记。EgyGene GelAnalyzer4提供了许多功能，在其他任何选择中可能无法一起使用。填充参数，增强图像的颜色和清晰度以及合并几种凝胶的数据。另外，在图像上写上样品名称和检测到的标记。EgyGene GelAnalyzer4提供了许多功能，在其他任何选择中可能无法一起使用。填充参数，增强图像的颜色和清晰度，并合并几种凝胶的数据。另外，在图像上写上样品名称和检测到的标记。EgyGene GelAnalyzer4提供了许多功能，在其他任何选择中可能无法一起使用。