A cryptic sexual reproductive cycle in Leishmania has been inferred through population genetic studies revealing the presence of hybrid genotypes in natural isolates, with attempts made to decipher sexual strategies by studying complex chromosomal inheritance patterns. A more informative approach is to study the products of controlled, laboratory-based experiments where known strains or species are crossed in the sand fly vector to generate hybrid progeny. These hybrids can be subsequently studied through high resolution sequencing technologies and software suites such as PAINT that disclose inheritance patterns including ploidies, parental chromosome contributions and recombinations, all of which can inform the sexual strategy. In this work, we discuss the computational methods in PAINT that can be used to interpret the sexual strategies adopted specifically by aneuploid organisms and summarize how PAINT has been applied to the analysis of experimental hybrids to reveal meiosis-like sexual recombination in Leishmania.

中文翻译：

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通过为非整倍体基因组设计的计算方法解决了利什曼原虫的性生殖策略

利什曼原虫中一个神秘的有性生殖周期已经通过群体遗传研究推断出自然分离株中存在杂种基因型，并试图通过研究复杂的染色体遗传模式来破译性策略。一个更有用的方法是研究受控的、基于实验室的实验的产物，在这些实验中，已知品系或物种在白蛉载体中杂交以产生杂交后代。随后可以通过高分辨率测序技术和软件套件（例如 PAINT）研究这些杂种，这些套件可以揭示遗传模式，包括倍性、亲本染色体贡献和重组，所有这些都可以为性策略提供信息。在这项工作中，利什曼原虫。