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Development and validation of a multi-residue analytical method for veterinarian and human pharmaceuticals in livestock urine and blood using UHPLC-QTOF
Journal of Chromatography B ( IF 3 ) Pub Date : 2021-01-26 , DOI: 10.1016/j.jchromb.2021.122564
Xue Wang , Ke-wen Wang , Zhen-zhen Xu , Shu-ming Yang , Yan Zhao

The aim of this work is to contribute to the assessment of multi-residue analysis of veterinarian and human pharmaceuticals using UHPLC-QTOF in livestock urine and blood (cattle, chicken, sheep and pig). Firstly, an in-house database including compound name, monoisotopic mass, chemical formula, retention time, chemical structure, and three CID MS-MS spectra of the 234 selected drugs were built for qualitative detection. Secondly, the method validation result showed that all the 234 drugs exhibited good linearity with determination coefficients (R2) higher than 0.999. Then, the distribution of the drugs recoveries, intra-day RSD and inter-day RSD results for all seven matrices were tested. Finally, after a carefully cross check, 150 veterinarian and human pharmaceuticals could meet the methodological requirements (recovery, 50–120%; intra-day RSD ≤ 15%, inter-day RSD ≤ 20%) in all seven matrices. Our results suggested that although the main applications of UHPLC-QTOF are directed towards detection and identification of the compounds, this method should be also applied for quantitative purposes.



中文翻译:

使用UHPLC-QTOF开发和验证牲畜尿液和血液中兽药和人用药物的多残留分析方法

这项工作的目的是有助于使用UHPLC-QTOF对家畜尿液和血液(牛,鸡,羊和猪)中的兽药和人用药物进行多残留分析的评估。首先,建立了一个内部数据库,其中包括化合物名称,单同位素质量,化学式,保留时间,化学结构和234种所选药物的3个CID MS-MS光谱,用于定性检测。其次,方法验证结果表明,所有234种药物的测定系数均具有良好的线性(R 2)高于0.999。然后,测试了所有七个基质的药物回收率分布,日内RSD和日间RSD结果。最后,经过仔细的交叉检查,在所有七个矩阵中,有150种兽医和人类药品可以满足方法要求(回收率50-120%;日内RSD≤15%,日间RSD≤20%)。我们的结果表明,尽管UHPLC-QTOF的主要应用是针对化合物的检测和鉴定,但该方法也应用于定量目的。

更新日期:2021-02-09
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