MnFe₂O₄ nanoparticles are used in medicine for cancer treatments through hyperthermia, gene, and drug delivery systems. The primary purpose of the current investigation was to study the effect of the MnFe₂O₄ nanoparticles on the structure and function of acid phosphatase. The enzyme activity displayed that MnFe₂O₄ nanoparticles acted as a non-competitive inhibitor. K_i was calculated to be 0.217 mM. The thermal denaturation analysis also demonstrated that T_m was decreased by adding MnFe₂O₄ nanoparticles concentrations (from 342.0–338.5 K). Far-UV circular studies also revealed that MnFe₂O₄ nanoparticles could alter the secondary structure of acid phosphatase by the enhancement of the \(\alpha\)-helix contents (from 10.8 to 11.3%) and the decrease of the \(\beta\)-sheet (from 30.2 to 29.8%). It was also shown that MnFe₂O₄ nanoparticles decreased the intrinsic fluorescence of acid phosphatase via the static quenching. Also, with the rise of temperature, the Stern–Volmer constant was declined from 9.95 \(\times {10}^{4}\) to 2.42 M\(\times {10}^{4}\)⁻¹. The hydrogen bonding and van der Waals interactions also played a significant role in this interaction. These results also showed that MnFe₂O₄ nanoparticles changed the conformational of acid phosphatase. Overall, MnFe₂O₄ nanoparticles could influence the activity and stability of acid phosphatase.

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中文翻译：

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MnFe 2 O 4纳米颗粒对酸性磷酸酶活性参数和稳定性影响的评价

MnFe ₂ O ₄纳米颗粒通过热疗，基因和药物输送系统用于医学领域，用于癌症治疗。当前研究的主要目的是研究MnFe ₂ O ₄纳米颗粒对酸性磷酸酶结构和功能的影响。酶活性表明，MnFe ₂ O ₄纳米颗粒起非竞争性抑制剂的作用。K _i计算为0.217 mM。热变性分析还表明，通过添加MnFe ₂ O _4可以降低T _m。纳米颗粒浓度（从342​​.0–338.5 K）。远紫外循环研究还表明，MnFe ₂ O ₄纳米颗粒可通过增加\（\ alpha \）-螺旋含量（从10.8％到11.3％）和降低\（\ beta \）-页（从30.2到29.8％）。还表明，MnFe ₂ O ₄纳米颗粒通过静态猝灭降低了酸性磷酸酶的固有荧光。另外，随着温度的升高，斯特恩-沃尔默常数从9.95 \（\ times {10} ^ {4} \）降至2.42 M \（\ times {10} ^ {4} \）- ¹。氢键和范德华相互作用在该相互作用中也起重要作用。这些结果还表明，MnFe ₂ O ₄纳米颗粒改变了酸性磷酸酶的构象。总体而言，MnFe ₂ O ₄纳米颗粒可影响酸性磷酸酶的活性和稳定性。

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