Background

Gastric cancer is one of the serious malignant tumors with high incidence worldwide. The two commonly used treatment strategies for gastric cancer include chemotherapy and radiation therapy. Conventional anticancer drugs have limited effectiveness. Therefore, it is important to explore new anticancer drugs. Dehydroabietic acid (DAA) is known to have anti-bacterial, anti-inflammatory, and anticancer effects. However, there are fewer reports on the effect of DAA on human gastric cancer cell lines.

Objective

We investigated the gastric cell growth inhibitory effects of DAA on human gastric cancer cell lines. Gastric cancer cell proliferation, apoptosis and cell cycle arrest assay were detected by WST assay, crystal violet staining assay, Flow cytometry, RT-PCR and western blot analysis.

Results

These results showed that as the concentration of DAA increased, the proliferation inhibitory effect appeared in gastric cancer cells. In addition, DAA induced sub-G1 phase accumulation and G1 cell cycle arrest, and subsequently induced apoptosis in AGS and YCC-2 cells. In addition, the expression of the pro-apoptotic Bax was upregulated, and that of the anti-apoptotic Bcl-2 was downregulated in DAA-treated AGS and YCC-2 cells. Moreover, DAA induced the cleavage of caspase-3 and PARP.

Conclusion

These results suggest that DAA induces cell death through mitochondria-mediated apoptosis and G1 cell cycle arrest. Therefore, our results indicate the anticancer and therapeutic potential of DAA for treatment of human gastric cancer.

中文翻译：

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脱氢松香酸通过诱导凋亡和细胞周期阻滞抑制胃癌细胞的生长

背景

胃癌是世界范围内高发的严重恶性肿瘤之一。胃癌的两种常用治疗策略包括化学疗法和放射疗法。常规的抗癌药效果有限。因此，重要的是探索新的抗癌药物。脱氢松香酸（DAA）已知具有抗菌，消炎和抗癌作用。然而，关于DAA对人胃癌细胞系的作用的报道较少。

目的

我们研究了DAA对人胃癌细胞系的胃细胞生长抑制作用。通过WST，结晶紫染色，流式细胞仪，RT-PCR和western blot分析检测胃癌细胞的增殖，凋亡和细胞周期阻滞。

结果

这些结果表明，随着DAA浓度的增加，在胃癌细胞中出现了增殖抑制作用。此外，DAA诱导亚G1期积累和G1细胞周期停滞，随后诱导AGS和YCC-2细胞凋亡。另外，在DAA处理的AGS和YCC-2细胞中，促凋亡Bax的表达被上调，而抗凋亡Bcl-2的表达被下调。此外，DAA诱导caspase-3和PARP的裂解。

结论

这些结果表明，DAA通过线粒体介导的凋亡和G1细胞周期阻滞诱导细胞死亡。因此，我们的结果表明DAA在治疗人胃癌方面具有抗癌和治疗潜力。