We report the pregnancy and live birth achieved after in vitro maturation (IVM) of oocytes and PGT-A in a 23-year-old patient suffering from ovarian gonadotropin resistance. A woman with resistant ovary syndrome (ROS) had secondary amenorrhea, high FSH levels (25.34 mIU/mL) and LH (29.6 mIU/mL), low estradiol levels (15.2 pg/mL), and high serum AMH levels (38.0 ng/mL), associated with an increased antral follicle count (AFC) of 45. Without gonadotropin priming and HCG trigger, ultrasound-guided transvaginal oocyte retrieval was performed. Aspiration of antral-stage follicles allowed the retrieval of 15 immature oocytes. After oocyte collection, immature oocytes were cultured in the IVM medium. Following IVM, six of them reached metaphase II stage. Resultant matured oocytes were fertilized by intracytoplasmic sperm injection (ICSI). Embryos obtained were cultured to the blastocyst stage. On day 5, three embryos reached blastocyst stage. Trophectoderm biopsy and PGT-A were performed on two better quality embryos on day 5 after fertilization. Two biopsied embryos were reported to be euploid. PGT-A was performed utilizing next-generation sequencing (NGS\MPS). One embryo was transferred in an artificial thaw cycle and resulted in a viable intrauterine pregnancy and live birth. Our experience indicates that there is no requirement for gonadotropin stimulation and use of b-hCG trigger prior to IVM in patients with ROS. The results suggest that oocytes obtained with IVM in patients with ROS are capable of meiotic and mitotic division, fertilization, and generation of euploid embryos. IVM appears to be a valuable approach in patients with ROS, allowing them to have genetically connected offspring.

我们报告了一名患有卵巢促性腺激素抵抗的 23 岁患者在卵母细胞和 PGT-A 体外成熟 (IVM) 后实现的妊娠和活产。一名患有抵抗性卵巢综合征 (ROS) 的女性出现继发性闭经，FSH 水平 (25.34 mIU/mL) 和 LH (29.6 mIU/mL) 水平高，雌二醇水平低 (15.2 pg/mL)，血清 AMH 水平高 (38.0 ng/mL) mL)，与增加的 45 的窦卵泡计数 (AFC) 相关。在没有促性腺激素启动和 HCG 触发的情况下，进行了超声引导的经阴道卵母细胞检索。胃窦阶段卵泡的抽吸允许回收 15 个未成熟的卵母细胞。卵母细胞收集后，未成熟卵母细胞在 IVM 培养基中培养。在IVM之后，其中六个达到了中期II阶段。通过胞浆内精子注射 (ICSI) 使所得成熟卵母细胞受精。获得的胚胎培养至囊胚期。第5天，三个胚胎达到囊胚期。在受精后第 5 天对两个质量较好的胚胎进行滋养外胚层活检和 PGT-A。据报道，两个活检胚胎是整倍体。PGT-A 是利用下一代测序 (NGS\MPS) 进行的。一个胚胎在人工解冻循环中被移植，并导致了可行的宫内妊娠和活产。我们的经验表明，对于 ROS 患者，在 IVM 之前不需要促性腺激素刺激和使用 b-hCG 触发器。结果表明，在 ROS 患者中通过 IVM 获得的卵母细胞能够进行减数分裂和有丝分裂、受精和整倍体胚胎的产生。IVM 似乎是治疗 ROS 患者的一种有价值的方法，