Scaevola taccada (Gaertn.) Roxb. is a hemi-sclerophyllous littoral shrub of the family Goodeniaceae. It is a salt-tolerant plant and used in soil reclamation and coastal landscaping programs to control beach erosion. The present article reports a reproducible in vitro regeneration system using meta-topolin (mT) through liquid medium employing nodal segment cultures. The highest number of axillary shoots (5.8 ± 0.29) was differentiated on Murashige and Skoog’s (MS) medium containing meta-topolin (1.5 mg L⁻¹). The amplification of shoots (49.8 ± 0.37) and fresh growth was achieved by recurrent subcultures on liquid MS medium supplemented with 0.25 mg L⁻¹ mT and 0.15 mg L⁻¹ indole-3-acetic acid (IAA) in the presence of additives (50 mg L⁻¹ ascorbic acid + 25 mg L⁻¹ citric acid, adenine sulphate and l-arginine). Rooting and acclimatization was simultaneously achieved using concurrent ex vitro rooting and acclimatization (CEVRA) technique by pulse treating the shoot base with 300 mg L⁻¹ IBA for 5 min. The in vitro developed shoots represented a maximum percentage (100%) of rooting in autoclaved soilrite in a greenhouse. After 2 mo, the plantlets were established in the field with cent percentage survival success. The genetic fidelity analysis using inter simple sequence repeats (ISSR) DNA molecular markers developed maximum of 55 monomorphic bands and revealed the genetic homogeneity of in vitro regenerated plantlets with the mother plant.

Scaevola taccada（Gaertn。）Roxb。是Goodeniaceae科的半硬毛的沿海灌木。它是一种耐盐植物，用于土壤开垦和沿海环境美化计划中，以控制海滩侵蚀。本文报道了一种可再生的体外再生系统，该系统使用间位拓扑蛋白（m T）通过采用节段培养的液体培养基进行培养。在含有间-topolin（1.5 mg L ^-1）的Murashige和Skoog's（MS）培养基上分化出最高数量的腋芽（5.8±0.29 ）。通过在补充有0.25 mg L ^-1 m的液体MS培养基上进行反复传代培养，可以实现枝条的扩增（49.8±0.37）和新鲜生长。在添加剂（50 mg L ^-1抗坏血酸+ 25 mg L ^-1柠檬酸，硫酸腺嘌呤和1-精氨酸）存在下，加入T和0.15 mg L ^-1吲哚-3-乙酸（IAA ）。通过同时用离体生根和驯化（CEVRA）技术同时进行生根和驯化，方法是用300 mg L ^-1 IBA脉冲处理芽基5分钟。在体外发达的芽代表了在温室中高压灭菌的土壤中生根的最大百分比（100％）。2个月后，在田间建立苗，存活百分比成功。使用内部简单序列重复（ISSR）DNA分子标记进行的遗传保真度分析显示出最多55个单态条带，并揭示了与母本植物体外再生的小植株的遗传同质性。