A quantitative analysis of ginsenoside is very important for ginseng studies because each ginsenoside shows different medical activity and metabolic pathway. In this study, a rapid, simultaneous, and quantitative analysis of 26 ginsenosides (Rb1, Rb2, Rc, Rd, Re, Rf, Rg1, Rg2(R), Rg2(S), Rg3(S), Rg3(R), Rg5, Rg6, Rh1(R), Rh1(S), Rh2(R), Rh2(S), F1, F2, F3, F4, K, Mc, PPT(S), XVII, and Y) in white, and red Panax ginseng was established using multiple reaction monitoring (MRM) mode on ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC–MS/MS). The mobile phase of water and methanol containing 0.1% formic acid and HSS T3 C18 analytical column was used for the chromatographic separation. The four sets of stereoisomers were successfully separated within a 26-min run time, eluting the S-isomer faster than the R-isomer with higher concentration. The ginseng extract was diluted by 100, 400 and 8000 times to fit in the calibration range and quantitated by the standard addition method. Matrix matched calibration by mixing 64 µL of the ginseng extract with 16 µL of the standard solution was used for compensating the matrix effect. Such quantitation methodology using dilution, standard addition and matrix matching resulted in precise and unambiguous quantitation of 26 ginsenosides in ginseng products. Major ginsenosides were observed at relatively higher concentrations in red Panax ginseng and the Mc was detected and quantitated for the first time in this study. The comprehensive quantitation system established in this study will contribute to quality evaluation, breeding and culturing, and quantitative metabolomics study of ginseng.

中文翻译：

---

在白色和红色26种人参皂甙的快速，同时和定量分析人参使用LC-MS / MS

人参皂苷的定量分析对于人参研究非常重要，因为每种人参皂苷显示出不同的医学活性和代谢途径。在这项研究中，对26种人参皂苷（Rb1，Rb2，Rc，Rd，Re，Rf，Rg1，Rg2（R），Rg2（S），Rg3（S），Rg3（R），白色的Rg5，Rg6，Rh1（R），Rh1（S），Rh2（R），Rh2（S），F1，F2，F3，F4，K，Mc，PPT（S），XVII和Y），以及在超高效液相色谱-串联质谱（UHPLC–MS / MS）上使用多反应监测（MRM）模式建立了红色人参。含有0.1％甲酸的水和甲醇的流动相和HSS T3 C18分析柱用于色谱分离。在26分钟的运行时间内成功分离了四组立体异构体，较高浓度的R-异构体洗脱S-异构体的速度更快。人参提取物分别稀释100倍，400倍和8000倍至校准范围，并通过标准添加方法进行定量。通过将64 µL人参提取物与16 µL标准溶液混合来进行基质匹配校准，以补偿基质效应。这种使用稀释，标准添加和基质匹配的定量方法论可以对人参产品中的26种人参皂甙进行精确而明确的定量。在该研究中首次观察到主要人参皂苷在红色人参中含量相对较高，并且首次检测并定量了Mc。本研究中建立的全面定量系统将有助于质量评估，育种和培养，