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Complete genome sequence of plant growth‐promoting and heavy metal‐tolerant Enterobacter tabaci 4M9 (CCB‐MBL 5004)
Journal of Basic Microbiology ( IF 3.1 ) Pub Date : 2021-01-25 , DOI: 10.1002/jobm.202000695
Saidu Abdullahi 1, 2 , Hazzeman Haris 1 , Kamarul Z Zarkasi 1 , Hamzah G Amir 1
Affiliation  

Enterobacter tabaci 4M9 (CCB-MBL 5004) was reported to have plant growth-promoting and heavy metal tolerance traits. It was able to tolerate more than 300 mg/L Cd, 600 mg/L As, and 500 mg/L Pb and still maintained the ability to produce plant growth-promoting substances under metal stress conditions. To explore the genetic basis of these beneficial traits, the complete genome sequencing of 4M9 was carried out using Pacific Bioscience (PacBio) sequencing technology. The complete genome consisted of one chromosome of 4,654,430 bp with a GC content of 54.6% and one plasmid of 51,135 bp with a GC content of 49.4%. Genome annotation revealed several genes involved in plant growth-promoting traits, including the production of siderophore, indole acetic acid, and 1-aminocyclopropane-1-carboxylate deaminase; solubilization of phosphate and potassium; and nitrogen metabolism. Similarly, genes involved in heavy metals (As, Co, Zn, Cu, Mn, Se, Cd, and Fe) tolerance were detected. These support its potential as a heavy metal-tolerant plant growth-promoting bacterium and a good genetic resource that can be employed to improve phytoremediation efficiency of heavy metal-contaminated soil via biotechnological techniques. This, to the best of our knowledge, is the first report on the complete genome sequence of heavy metal-tolerant plant growth-promoting E. tabaci.

中文翻译:

植物生长促进和重金属耐受性烟粉虱肠杆菌 4M9 (CCB-MBL 5004) 的完整基因组序列

据报道,烟粉肠杆菌​​ 4M9 (CCB-MBL 5004) 具有促进植物生长和重金属​​耐受性的特性。它能够耐受超过 300 mg/L 的 Cd、600 mg/L 的 As 和 500 mg/L 的 Pb,并且在金属胁迫条件下仍然保持产生植物生长促进物质的能力。为了探索这些有益性状的遗传基础,使用太平洋生物科学(PacBio)测序技术对4M9进行了全基因组测序。完整的基因组由一条 4,654,430 bp 的染色体组成,GC 含量为 54.6%,一个质粒长度为 51,135 bp,GC 含量为 49.4%。基因组注释揭示了几个与植物生长促进性状有关的基因,包括铁载体、吲哚乙酸和 1-氨基环丙烷-1-羧酸脱氨酶的产生;溶解磷酸盐和钾;和氮代谢。同样,检测到参与重金属(As、Co、Zn、Cu、Mn、Se、Cd 和 Fe)耐受性的基因。这些支持其作为耐重金属植物生长促进细菌和良好遗传资源的潜力,可用于通过生物技术技术提高重金属污染土壤的植物修复效率。据我们所知,这是第一份关于耐重金属植物促生长烟粉虱的完整基因组序列的报告。这些支持其作为耐重金属植物生长促进细菌和良好遗传资源的潜力,可用于通过生物技术技术提高重金属污染土壤的植物修复效率。据我们所知,这是第一份关于耐重金属植物促生长烟粉虱的完整基因组序列的报告。这些支持其作为耐重金属植物生长促进细菌和良好遗传资源的潜力,可用于通过生物技术技术提高重金属污染土壤的植物修复效率。据我们所知,这是第一份关于耐重金属植物促生长烟粉虱的完整基因组序列的报告。
更新日期:2021-01-25
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