Our aim was to verify whether genes in the coagulation complement and cytokine interaction pathways are crucial to the resistance to grass carp hemorrhagic disease in grass carps (Ctenopharyngodon idella). To evaluate this, we acquired male grass carps with grass carp reovirus (GCRV) resistance, F1 generation, ordinary male fish, control F1 progenies (hybridized ordinary fish), and barbel chub (Squaliobarbus curriculus) as a positive control. We compared the expression variation of 13 immune factors in different tissues using real-time fluorescent quantitative PCR. Our results showed that the expression of the transforming growth factor-β3, nerve growth factor receptor, coagulation factor D, chemokine receptor XCR1, profibrinolysin, γ-globulin of complement component 1, complement component 2, and complement component 5 in the different tissues of the barbel chub were significantly higher than those in the grass carps populations under normal conditions. Except for a limited number of genes, their expression exhibited significant differences. There was no significant difference in these genes among grass carp populations with different GCRV resistance. These results indicate that the high gene expression in the coagulation complement and cytokine interaction pathways may be the main reason for the strong resistance of barbel chub to GCRV. However, high gene expression was not a factor affecting GCRV resistance between grass carp populations.

我们的目的是验证凝血补体和细胞因子相互作用途径中的基因对于草鱼（Ctenopharyngodon idella）对草鱼出血性疾病的抗性是否至关重要。为了对此进行评估，我们获得了具有草re呼肠孤病毒（GCRV）抗性，F1代，普通雄鱼，对照F1后代（杂交普通鱼）和Barbel chub（Squaliobarbus curriculus）的雄性草鱼。）作为阳性对照。我们使用实时荧光定量PCR比较了13种免疫因子在不同组织中的表达变化。我们的研究结果表明补体成分1，补体成分2和补体成分5中转化生长因子-β3，神经生长因子受体，凝血因子D，趋化因子受体XCR1，纤溶酶，γ球蛋白在不同组织中的表达在正常条件下，通天明显高于草鱼种群。除了有限数量的基因，它们的表达表现出显着差异。这些基因在具有不同GCRV抗性的草鱼种群之间没有显着差异。这些结果表明，凝血补体和细胞因子相互作用途径中的高基因表达可能是Barbel chub对GCRV产生强抗性的主要原因。然而，高基因表达不是影响草鱼种群之间GCRV抗性的因素。