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Ethanolamine Plasmalogen Suppresses Apoptosis in Human Intestinal Tract Cells in Vitro by Attenuating Induced Inflammatory Stress
ACS Omega ( IF 4.1 ) Pub Date : 2021-01-22 , DOI: 10.1021/acsomega.0c05545
Ephantus Nguma 1 , Shinji Yamashita 1 , Kei Kumagai 2 , Yurika Otoki 2 , Ayaka Yamamoto 3 , Takahiro Eitsuka 2 , Kiyotaka Nakagawa 2 , Teruo Miyazawa 4 , Mikio Kinoshita 1
Affiliation  

Ethanolamine plasmalogen (PlsEtn) is a subtype of ethanolamine glycerophospholipids (EtnGpl). Recently, PlsEtn has attracted increasing research interest due to its beneficial effects in health and disease; however, its functional role in colonic health has not been well established. This study was conducted to determine the mechanism underlying the antiapoptotic effect of PlsEtn in human intestinal tract cells under induced inflammatory stress. Lipopolysaccharide induced apoptosis of differentiated Caco-2 cells, which was suppressed by EtnGpl in a dose-dependent manner. Cells treated with ascidian muscle EtnGpl containing high levels of PlsEtn demonstrated a lower degree of apoptosis, and downregulated TNF-α and apoptosis-related proteins compared to those treated with porcine liver EtnGpl containing low PlsEtn. This indicates that PlsEtn exerted the observed effects, which provided protection against induced inflammatory stress. Overall, our results suggest that PlsEtn with abundant vinyl ether linkages is potentially beneficial in preventing the initiation of inflammatory bowel disease and colon cancer.

中文翻译:

乙醇胺缩醛禁止显示凋亡的人体肠道细胞在体外通过减弱引起的炎症压力

乙醇胺缩醛磷脂(PlsEtn)是乙醇胺甘油磷脂(EtnGpl)的亚型。最近,由于其对健康和疾病的有益作用,PlsEtn引起了越来越多的研究兴趣。然而,其在结肠健康中的功能作用尚未得到很好的确立。进行了这项研究,以确定诱导的炎症应激下PlsEtn对人肠道细胞抗凋亡作用的潜在机制。脂多糖诱导分化的Caco-2细胞凋亡,被EtnGpl抑制,呈剂量依赖性。与用低PlsEtn的猪肝EtnGpl处理的细胞相比,用含有高水平的PlsEtn的海鞘肌肉EtnGpl处理的细胞显示出较低的凋亡程度,并下调了TNF-α和凋亡相关蛋白。这表明PlsEtn发挥了观察到的作用,为诱导的炎症应激提供了保护。总体而言,我们的结果表明,具有丰富的乙烯基醚键的PlsEtn可能对预防炎症性肠病和结肠癌的发生具有潜在的益处。
更新日期:2021-02-02
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